豆科植物查尔酮合成酶基因密码子偏好性分析

发布时间：2018-05-18 14:25

本文选题：豆科植物 + 查尔酮合成酶　；参考：《华北农学报》2016年06期

【摘要】：查尔酮合成酶(Chalcone synthase,CHS)是参与合成植物苯丙烷类物质的主要酶类之一。研究豆科植物CHS基因密码子的偏好性,对提高豆科植物CHS基因的表达水平具有重要意义。为此,运用CHIPS、CUSP和Codon W程序分析豆科植物CHS基因密码子的偏好性,并用决明CHS基因对分析结果的可信度进行验证。结果表明,在豆科植物CHS基因的密码子中,以A或U结尾的密码子偏好性较强。基于CHS基因RSCU值的聚类结果显示,豆科植物与茄科植物(包括烟草)聚为一类,表明烟草作为研究豆科植物CHS基因功能的模式植物较为合适。通过对决明CHS基因密码子偏好性与大肠杆菌和酵母基因组密码子偏好性的比较,发现两者均存在差异,但酵母的差异低于大肠杆菌,表明酵母表达系统更加适合作为决明CHS基因的外源表达系统。然而,若要使决明CHS基因能够在酵母表达系统中高效表达,仍需对其密码子进行优化。
[Abstract]:Chalcone synthase CHS (Chalcone synthase) is one of the main enzymes involved in the synthesis of phytopropanes. It is important to study the preference of CHS gene codon in legume plants to improve the expression level of CHS gene. Therefore, the preference of CHS codon in legume plants was analyzed by using CHIPSU CUSP and Codon W program, and the reliability of the results was verified by using the CHS gene of Cassia. The results showed that the codon at the end of A or U was stronger in the codon of CHS gene in Leguminosae. The clustering results based on the RSCU value of CHS gene showed that legume and eggplant (including tobacco) were clustered together, which indicated that tobacco was more suitable as a model for studying the function of CHS gene in legume. By comparing the codon preference of CHS gene with Escherichia coli and yeast genome codon preference, it was found that there were differences between them, but the difference between yeast and Escherichia coli was lower than that of Escherichia coli. The results showed that yeast expression system was more suitable for exogenous expression of CHS gene. However, in order to efficiently express the CHS gene in yeast, the codon should be optimized.
【作者单位】：西南交通大学生命科学与工程学院;
【基金】：国家自然科学基金项目(31371232;31500276) 成都市科技技术研发项目(2015-HM01-00051-SF) 西南交通大学校级大学生创新创业训练计划资助项目(151607)
【分类号】：Q943.2

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