5个绵羊品种CAST基因多态性及其与肉品质的相关性

发布时间：2018-05-19 10:57

本文选题：绵羊 + PCR-SSCP　；参考：《浙江农业学报》2016年08期

【摘要】：为从分子水平研究甘肃境内典型养殖模式下甘肃高山细毛羊、小尾寒羊、藏羊、蒙古羊和滩羊5个绵羊品种的CAST基因多态性与肉品质的相关性,探讨以CAST为候选基因,提高绵羊肉品质的可能性。采用PCR-SSCP技术对5个绵羊品种的CAST基因内含子3多态性进行检测,并就CAST基因多态性与9月龄肉品质进行相关性分析。根据比对结果发现,与等位基因A相比,等位基因B发现G62T以及C110T突变,等位基因C发现C92T突变,形成AA、AB、BB和AC 4种基因型;经检验表明,5个绵羊品种均处于非Hardy-Weinberg平衡状态;群体遗传多态性分析表明,5个绵羊品种的多态信息含量均为中度多态;关联分析表明,5个绵羊品种CAST基因变异位点的4个基因型间失水率AC基因型显著高于其他基因型(P0.05),对于剪切力AC基因型显著低于其他3种基因型(P0.05);不同绵羊品种间基因型分析表明,对于5个绵羊品种AC基因型的失水率显著高于其他3种基因型,除蒙古羊外,AC基因型的剪切力显著低于其他基因型;变异对眼肌面积没有显著性影响。这些关联说明CAST基因可作为候选基因用于绵羊产肉性能和肉品质的分子标记辅助选择。
[Abstract]:In order to study the relationship between CAST gene polymorphisms and meat quality of Gansu alpine fine wool sheep, small tail Han sheep, Tibetan sheep, Mongolian sheep and Tan sheep under typical breeding model in Gansu Province, CAST was used as candidate gene in order to study the relationship between the polymorphism of CAST gene and meat quality of Gansu alpine sheep, small tail Han sheep, Tibetan sheep, Mongolian sheep and Tan sheep. The possibility of improving the meat quality of sheep. PCR-SSCP technique was used to detect intron 3 polymorphism of CAST gene in five sheep breeds, and the correlation between CAST gene polymorphism and meat quality at 9 months was analyzed. Compared with allele A, allele B found G62T and C110T mutation, allele C found C92T mutation, forming four genotypes of AAABA BB and AC, the results showed that all five sheep breeds were in non-Hardy-Weinberg equilibrium state. Population genetic polymorphism analysis showed that the polymorphic information content of the five sheep breeds was moderately polymorphic. The correlation analysis showed that the water loss rate among the four genotypes of the five sheep breeds with CAST gene mutation sites was significantly higher than that of the other genotypes P0.05A, and the AC genotype with shear stress was significantly lower than the other three genotypes (P0.05N), and different sheep breeds with different sheep breeds. The genotype analysis showed that, The water loss rate of AC genotype in five sheep breeds was significantly higher than that of the other three genotypes, and the shear stress of AC genotype was significantly lower than that of other genotypes except Mongolian sheep, and the variation had no significant effect on eye muscle area. These associations suggest that CAST gene can be used as a candidate gene for molecular marker-assisted selection of meat performance and meat quality in sheep.
【作者单位】：甘肃农业大学动物科学技术学院;
【基金】：甘肃省农业生物技术研究与应用开发项目(GNSW-2012-26)
【分类号】：S826

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