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小麦新品种鄂麦170抗病基因的遗传分析

发布时间:2018-05-19 16:37

  本文选题:小麦 + 鄂麦 ; 参考:《分子植物育种》2017年09期


【摘要】:通过已开发分子标记对湖北省有潜力的新品种鄂麦170及其双亲的抗赤霉病、条锈病、白粉病和纹枯病基因进行分子鉴定和分析。结果表明,鄂麦170含有3个主效抗赤霉病基因(Fhb1,Fhb5和以Xwmc273为标记的基因)、3个抗条锈病基因(Yr2,Yr5和Yr26)、8个抗白粉病基因(Pm2,Pm8,Pm16,Pm21,Pm31,Pm33,Pm35和Pm36)和6个抗纹枯病基因(分别以Xwmc397,Xgwm212,Xgdm67,Xwmc497,Xgwm644和Xgwm526为标记)。经分析鄂麦170中可能还有未被检测到的赤霉病抗性位点,而鄂麦170赤霉病抗性优于双亲可能与超亲优势有关;Yr5基因可能对鄂麦170的抗条锈病特性起重要作用;抗白粉病基因Pm21已表现出了感病特征;所检测到的纹枯病抗性位点的表型变异解释率较低。本研究解释了鄂麦170的抗病分子基础,为该品种的推广应用和作为骨干亲本培育多抗小麦新品种提供了参考。
[Abstract]:The genes resistant to scab, stripe rust, powdery mildew and sheath blight were identified and analyzed by using molecular markers. The results showed that Emai 170 contained three major scab resistant genes, Fhb1, Fhb5 and Xwmc273 marker, three stripe rust resistant genes, Yr2Yr5 and Yr26, eight powdery mildew resistant genes, Pm16Pm21, Pm31Pm33Pm35 and Pm36, and six sheath blight resistant genes (Xwmc397Xgwm212Xgdm67Xm497Xwmc497Xgwm644 and Xgwm526). It was analyzed that there may also be scab resistance sites not detected in Emai 170, and that the resistance to scab of Emai 170 is superior to that of its parents, and that Yr5 gene may play an important role in the resistance to stripe rust of Emai 170. The resistance gene of powdery mildew (Pm21) has been shown to be susceptible to the disease, and the interpretation rate of phenotypic variation of the resistance sites to Rhizoctonia solani is low. This study explained the molecular basis of Emai 170 disease resistance and provided a reference for the popularization and application of this variety and the breeding of new multi-resistant wheat varieties as backbone parents.
【作者单位】: 湖北省农业科学院粮食作物研究所农业部华中地区小麦病害生物学科学观测实验站湖北省小麦工程技术研究中心;湖北省农业科学院植保土肥研究所;主要粮食作物产业化湖北省协同创新中心;
【基金】:转基因生物新品种培育科技重大专项(2014ZX0800202B-003) 湖北省自然科学基金(2016CFB522) 国家小麦产业技术体系“武汉综合试验站”(CARS-03) 湖北省农业科学院青年基金(2016NKYJJ03)共同资助
【分类号】:S435.121.4

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