沙棘PEPCK基因的克隆及表达研究

发布时间：2018-05-20 11:38

本文选题：沙棘 + PEPCK基因　；参考：《西北植物学报》2017年10期

【摘要】：以沙棘为研究对象,通过RACE、基因克隆和定量PCR技术,克隆并分析沙棘磷酸烯醇式丙酮酸羧激酶基因(PEPCK)的序列信息,以及进化树构建和氨基酸的二级结构分析,比较NaCl胁迫后PEPCK基因的表达变化,为揭示PEPCK基因在沙棘抗性方面的功能研究奠定基础。结果显示:(1)成功克隆了沙棘PEPCK基因,该基因开放阅读框(ORF)为2 001bp,编码666个氨基酸。(2)序列分析表明,沙棘PEPCK与蓖麻、黄顶菊、核桃等序列一致性高达85%以上;进化上沙棘PEPCK基因与蓖麻和狭叶羽扇豆较近,与烟草、拟南芥进化关系较远。(3)在300mmol·L~(-1) NaCl盐胁迫下,沙棘PEPCK基因表达在胁迫组中显著上升,胁迫后7d时为未处理的2.5倍,15d时达到未处理的3.2倍。研究表明,沙棘PEPCK基因在沙棘适应外源盐胁迫的过程中可能发挥了重要作用。
[Abstract]:Hippophae rhamnoides (Hippophae rhamnoides) was used to clone and analyze the sequence information of phosphoenolpyruvate carboxykinase gene (PEPCK), the construction of phylogenetic tree and the secondary structure of amino acids by race, gene cloning and quantitative PCR. To study the function of PEPCK gene in Hippophae rhamnoides resistance by comparing the expression of PEPCK gene after NaCl stress. The results showed that PEPCK gene of Hippophae rhamnoides was cloned successfully. The ORF of the gene was 2 001 BP, encoding 666 amino acids. The sequence analysis showed that the sequence consistency of seabuckthorn PEPCK with ricin, yellow top and walnut was more than 85%. The PEPCK gene of Hippophae rhamnoides was close to ricin and narrow leaf lupin, but it was far related to tobacco and Arabidopsis thaliana. The expression of PEPCK gene in Hippophae rhamnoides increased significantly under the stress of NaCl salt stress. At 7 days after stress, it was 2.5 times as much as untreated and 3.2 times as much as untreated at 15 days. The results showed that the PEPCK gene of Hippophae rhamnoides may play an important role in the adaptation of Hippophae rhamnoides to exogenous salt stress.
【作者单位】：新疆农业大学林学与园艺学院;阿克苏地区林业科学研究所;新疆师范大学;
【基金】：自治区自然科学基金项目(2014211B037)
【分类号】：Q943.2;S793.6

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