十字花科黑腐病菌一个编码FhrR蛋白的基因功能分析

发布时间：2018-05-20 13:26

  本文选题：十字花科黑腐病菌 + rpf/DSF系统　； 参考：《基因组学与应用生物学》2017年08期

【摘要】：十字花科黑腐病菌(Xanthomonas campestris pv.campestris,Xcc)的Rpf C/Rpf G双组分系统感应DSF(diffusible signal factor)因子,并通过二级信使环二鸟苷酸(c-di-GMP)调控下游Clp、Zur和FhrR等全局性转录因子。为进一步研究Fhr R在Xcc中的调控作用,本研究构建了编码Fhr R蛋白的XC3027基因的整合突变体PK3027。植株试验表明PK3027突变对致病力和过敏(HR)反应没有影响。将连有hrpG、hrpX、hrp B、hrc U和hrp F启动子的p L6GUS报告质粒导入PK3027中,在XCM培养基中检测gus报告基因表达,结果表明XC3027对这些hrp基因的表达没有影响。表达纯化Fhr R蛋白后进行了凝胶阻滞实验,发现该转录调控蛋白不能直接与hrp X启动子结合。在Xcc8004中FhrR不参与Ⅲ型分泌系统相关基因的调控,并与病原菌致病无关。
[Abstract]:The Rpf C/Rpf G two-component system of Xanthomonas campestris pv.campestris Xcc) induces the DSF(diffusible signal factor-factor, and regulates the local transcription factors such as Clpsil-Zur and FhrR through the secondary messenger cyclic guanosine monophosphate (c-di-GMP) in the lower reaches of Xanthomonas campestris pv.campestris Xcc. In order to further study the regulatory role of Fhr R in Xcc, an integrated mutant PK302727 of XC3027 gene encoding Fhr R protein was constructed. Plant tests showed that PK3027 mutation had no effect on pathogenicity and hypersensitivity. The expression of gus reporter genes was detected in XCM medium. The results showed that XC3027 had no effect on the expression of these hrp genes. After expression and purification of Fhr R protein, gel arrest assay was carried out. It was found that the transcription regulatory protein could not directly bind to hrp X promoter. FhrR is not involved in the regulation of type 鈪，

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