内镜保胆胆囊组织结石复发易感基因调控研究

发布时间：2018-05-21 18:16

本文选题：易感基因 + 高通量测序　；参考：《中国内镜杂志》2017年07期

【摘要】：目的采用高通量测序技术通过对胆囊结石复发患者胆囊黏膜中基因表达谱的检测及生物信息学的分析,初步了解基因在胆囊结石发生发展中的作用机制。方法用Illumina Hi Seq 2500新一代高通量测序技术对7例胆囊结石及2例胆囊息肉复发患者进行mRNA表达谱差异筛选分析,并对得到的数据进行Gene Ontology(GO)和京都基因与基因组(KEGG)显著性富集统计。结果高通量测序结果显示,差异表达的mRNA差异基因共有150条。通过GO功能显著性富集分析,发现分子功能的GO功能显著富集是抗原结合;而对于生物过程,富集的GO功能是免疫应答;对于细胞成分,富集的GO功能是细胞外区域。KEGG通路富集分析发现最显著的途径是细胞因子-细胞因子受体作用途径(P=0.000)。结论目前的研究表明,研究胆囊结石复发易感基因,可以为预防内镜保胆术后胆囊结石复发的科学研究工作及临床治疗提供一个重要的参考。
[Abstract]:Objective to investigate the mechanism of gene expression in gallbladder gallstone by high-throughput sequencing technique by detecting gene expression profile and bioinformatics analysis in gallbladder mucosa of patients with gallstone recurrence. Methods Illumina Hi Seq 2500 high-throughput sequencing technique was used to screen and analyze the differential expression profiles of mRNA in 7 patients with gallstone and 2 patients with recurrent polyps of gallbladder. The data obtained were analyzed by Gene Ontology (GOG) and the significant enrichment statistics of Kyoto gene and genomic KEGG. Results High throughput sequencing showed that there were 150 differentially expressed mRNA genes. Through the analysis of go functional significance enrichment, it is found that the go function enrichment of molecular function is antigen binding, while for biological process, the enriched go function is immune response. Enriched go function is extracellular region. KEGG pathway enrichment analysis found that the most significant pathway is the cytokine-cytokine receptor pathway Pu 0.000. Conclusion the present study indicates that the study of the susceptible gene of gallstone recurrence can provide an important reference for the scientific research and clinical treatment of preventing the recurrence of gallstone after endoscopic cholecystectomy.
【作者单位】：河北省保定市第二医院肝胆外科;
【分类号】：R575.62

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