糖尿病大鼠P-糖蛋白编码基因Abcb1 mRNA的表达及其对口服恩诺沙星药动学的影响

发布时间：2018-05-22 08:22

本文选题：P-糖蛋白编码基因Abcb + 大鼠　；参考：《江苏农业学报》2017年04期

【摘要】：试验旨在研究糖尿病大鼠肝脏、肾脏、空肠和回肠中P-糖蛋白编码基因Abcb1 mRNA的表达水平及其对口服恩诺沙星药动学的影响。通过链脲佐菌素(STZ)诱导建立清洁级雄性Sprague-Dawley(SD)大鼠的糖尿病模型,采用实时荧光定量法检测并比较2组(健康组和糖尿病组)大鼠不同器官中Abcb1 mRNA的表达差异,采用高效液相色谱法(HPLC)进一步测定糖尿病大鼠口服恩诺沙星的血药浓度。结果表明,糖尿病大鼠肝脏和回肠中Abcb1a mRNA的表达量显著高于健康组(P0.05),肾脏和空肠中Abcb1a mRNA的表达量虽有波动,但差异不显著(P0.05)。健康大鼠各器官中Abcb1b mRNA的表达水平与糖尿病大鼠相比未见显著差异(P0.05)。口服恩诺沙星的药动学结果表明,糖尿病组大鼠血液中恩诺沙星的峰浓度、0~12 h的药-时曲线下面积和表观分布容积与健康组大鼠相比,均发生显著变化(P0.01、P0.05、P0.05),其他药动学参数虽有变化,但差异不显著(P0.05)。推测,糖尿病会导致大鼠体内Abcb1a mRNA的表达量发生变化,进一步导致口服药物在大鼠体内药动学过程发生变化。
[Abstract]:The aim of this study was to investigate the expression of Abcb1 mRNA in liver, kidney, jejunum and ileum of diabetic rats and its effect on oral enrofloxacin pharmacokinetics. The diabetic model of clean male Sprague-Dawley SD rats was established by streptozotocin (STZ). The expression of Abcb1 mRNA in different organs of two groups (healthy group and diabetic group) was detected and compared by real-time fluorescence quantitative method. The serum concentration of enrofloxacin in diabetic rats was further determined by HPLC. The results showed that the expression of Abcb1a mRNA in liver and ileum of diabetic rats was significantly higher than that in healthy control group (P 0.05). Although the expression of Abcb1a mRNA in kidney and jejunum fluctuated, the difference was not significant. There was no significant difference in the expression of Abcb1b mRNA between healthy rats and diabetic rats (P 0.05). The pharmacokinetic results of oral enrofloxacin showed that the peak concentration of enrofloxacin in blood of diabetic rats was less than that of healthy rats. There were significant changes in P0.01P0.05P0.05 and other pharmacokinetic parameters, but the difference was not significant. It is speculated that diabetes may cause changes in the expression of Abcb1a mRNA in rats and further changes in pharmacokinetics of oral drugs in rats.
【作者单位】：南京农业大学动物医学院;
【基金】：国家重点研发计划(2016YFD0501309) 江苏省高校“青蓝工程”中青年学术带头人项目(2014)
【分类号】：S859.7

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