陆地棉PPO基因全基因组鉴定及对黄萎病菌的响应分析

发布时间：2018-05-22 20:51

本文选题：陆地棉 + 多酚氧化酶　；参考：《棉花学报》2017年05期

【摘要】：【目的】多酚氧化酶(Polyphenol oxidases,PPO)广泛参与植物抵抗病虫害等生物逆境胁迫的过程。本研究旨在研究棉花中的PPO基因及其表达模式,验证多酚氧化酶与棉花抗黄萎病的关系。【方法】分析了黄萎病菌V991侵染下异源四倍体陆地棉TM-1根系中PPO酶活力变化,并利用TM-1的基因组数据库,鉴定相关基因,并进行生物信息学和表达分析。【结果】共筛选到13个候选GhPPO基因。这些基因都不含内含子,所编码的蛋白包含2个铜离子结合位点和PPO的保守序列(酪氨酸酶、DWL和KFDV保守结构域)。进化分析显示,陆地棉PPO基因的种内相似性大于种间相似性,并且相互之间形成了多对重复基因。GhPPO的表达量差异较大,少数基因具有组织特异表达的特性,多数基因在棉花不同组织中表达量都很低。实时荧光定量聚合酶链式反应分析结果表明:GhPPO6D在棉花根系中表达量较高,且在黄萎病菌V991侵染后上调。【结论】GhPPO6D可能参与了棉花与黄萎病菌的互作过程,与V991侵染后棉花根系PPO酶活力上升相关。
[Abstract]:[objective] Polyphenol oxidase (PPO) is widely involved in plant resistance to plant disease and insect pests and other biological stresses. The purpose of this study was to study the PPO gene and its expression pattern in cotton, and to verify the relationship between polyphenol oxidase and cotton resistance to Verticillium wilt. [methods] the changes of PPO enzyme activity in TM-1 roots of allotetraploid upland cotton infected with Verticillium wilt strain V991 were analyzed. Using the genomic database of TM-1, the related genes were identified, and bioinformatics and expression analysis were carried out. [results] Thirteen candidate GhPPO genes were screened out. These genes contain no introns, and the encoded proteins contain two copper ion binding sites and conserved sequences of PPO (tyrosinase DWL and KFDV conserved domain). Evolutionary analysis showed that the intraspecific similarity of PPO gene in upland cotton was greater than that among species, and the expression of multiple pairs of repeat genes. A few of them had the characteristics of tissue-specific expression. The expression of most genes was very low in different tissues of cotton. The results of real-time fluorescence quantitative polymerase chain reaction showed that the expression of 1: GhPPO6D was higher in cotton roots and up-regulated after infection with Verticillium wilt strain V991. [conclusion] GhPPO6D may be involved in the interaction between cotton and Verticillium wilt. It was related to the increase of PPO enzyme activity in cotton roots after V991 infection.
【作者单位】：河南大学生命科学学院/棉花生物学国家重点实验室/河南省植物逆境生物学重点实验室;
【基金】：棉花生物学国家重点实验室开放课题(CB2015A31,CB2016A23)
【分类号】：S435.62

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