管氏肿腿蜂寄生对黄粉甲硫氧还蛋白基因转录的影响

发布时间：2018-05-23 20:13

本文选题：黄粉甲 + 硫氧还蛋白　；参考：《西南林业大学学报》2016年06期

【摘要】：硫氧还蛋白参与体内必要的抗氧化作用和氧化还原调控过程,在昆虫体内能维持稳定的氧化还原状态,为初步了解硫氧还蛋白在黄粉甲体内的作用以及管氏肿腿蜂寄生对该蛋白基因转录的影响,采用RACE技术,克隆获得黄粉甲硫氧还蛋白基因,并进行多序列比对及荧光定量PCR分析。结果表明:cDNA序列长531 bp,开放阅读框318 bp,其推导的氨基酸序列编码106个氨基酸,3'端非编码区序列为95 bp,5'端非编码区序列为119 bp;预测理论分子量为11.97 kDa,等电点为4.22,含有一个保守的氧化还原活性位点CGPC。黄粉甲硫氧还蛋白基因编码的蛋白序列与其他昆虫的硫氧还蛋白相似性高于60%;硫氧还蛋白基因在黄粉甲各发育阶段中,在蛹期的表达水平最高,在成虫期表达水平最低,在蛹期时的脂肪体中高度表达。被管氏肿腿蜂寄生后,黄粉甲硫氧还蛋白基因转录水平明显受到上调诱导。
[Abstract]:Thioredoxin participates in the essential anti-oxidation and redox regulation process in vivo and can maintain a stable redox state in insects. In order to understand the role of thioredoxin in yellow powder and the effect of parasitism on the transcription of thioredoxin gene by Coprinus tubuli, the gene was cloned by RACE. Multiple sequence alignment and fluorescence quantitative PCR analysis were performed. The results show that the length of the 1: cDNA is 531 BP, the open reading frame is 318 BP, the deduced amino acid sequence encodes 106 amino acids with a 3 '-terminal noncoding region of 95 BP, the predicted molecular weight is 11.97 kDa, the isoelectric point is 4.22, and the deduced amino acid sequence is 95 BP / 5' -terminal non-coding region sequence, with a predicted molecular weight of 11.97 kDa and an isoelectric point of 4.22. There is a conserved redox active site CGPC. The similarity of thioredoxin gene with other insects was higher than that of other insects, and the expression level of thioredoxin gene was the highest in the pupal stage and the lowest in the adult stage. It is highly expressed in the fat body during the pupa. The transcription level of methylthioredoxin gene was upregulated after parasitized by Coprinus tumestris.
【作者单位】：西南林业大学云南省森林灾害预警与控制重点实验室;云南省热带作物科学研究所;
【基金】：国家自然科学基金项目(31260449)资助云南省林学一级学科博士点建设项目(林学学位授权学科项目经费)资助云南省中青年学术与技术带头人后备人才项目(2013HB077)资助云南省高校森林有害生物科技创新团队项目资助
【分类号】：S763.306.4

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