SALL2基因在口腔肿瘤中的表达及作用的研究

发布时间：2018-05-24 03:18

本文选题：口腔肿瘤 + 婆罗双树样基因2　；参考：《安徽医科大学》2017年硕士论文

【摘要】：目的婆罗双树样基因2(SALL2)是一种肿瘤抑制基因,具有抑制肿瘤细胞生长,促使肿瘤细胞凋亡的作用。检测SALL2基因在口腔肿瘤中的表达情况,研究其对口腔肿瘤细胞增殖、迁移能力的影响,探讨SALL2是否可作为口腔肿瘤标志物,在口腔肿瘤的治疗过程中发挥作用。方法构建SALL2基因过表达及敲低的载体,并将载体导入到口腔肿瘤(SCC-3)细胞株中,检测口腔肿瘤细胞株中SALL2基因的表达情况。通过MTT实验、细胞划痕实验观察细胞增殖、迁移的变化;收集15组口腔肿瘤组织与瘤旁组织,应用QRT-PCR法检测组织中SALL2 m RNA的表达。结果1.设计并合成含有目的基因的质粒,并将质粒通过慢病毒包装的方法导入口腔肿瘤细胞,通过此方法可构建稳定过表达或基因敲低的口腔肿瘤细胞株;2.通过划痕实验结果比较各组SCC-3细胞株向划痕中间生长移动的速度,SALL2基因的过表达具有抑制口腔肿瘤细胞迁移的作用,而敲低SALL2基因具有增强口腔肿瘤细胞迁移的作用。3.通过MTT实验检测各组SCC-3细胞株的生长曲线,SALL2基因的过表达具有抑制口腔肿瘤细胞增殖的作用,而敲低SALL2基因具有增强口腔肿瘤细胞增殖的作用。4.QRT-PCR法对15组标本组织中SALL2 m RNA的表达水平进行检测,表明在口腔肿瘤组织中,SALL2的表达水平相较于正常组织呈减少趋势。结论1.SALL2参与口腔肿瘤的发生及发展,通过一定的机制对口腔肿瘤细胞周期的调控,抑制肿瘤细胞的迁移和生长。2.SALL2可以作为口腔肿瘤的较有潜力的肿瘤分子标记物来判定肿瘤细胞的生物学行为,监测肿瘤的转移,评估治疗效果以及预后提供新的思路。3.SALL2在口腔肿瘤组织中表达的调节机制,生物学功能以及抑制肿瘤转移的具体机制尚未阐明,本研究为进一步探讨SALL2在口腔肿瘤中的作用机制提供了一定的理论基础。
[Abstract]:Objective Borneo double tree like gene 2pSALL2 is a tumor suppressor gene, which can inhibit the growth of tumor cells and promote apoptosis of tumor cells. To detect the expression of SALL2 gene in oral tumor, to study its effect on the proliferation and migration of oral tumor cells, and to explore whether SALL2 can be used as a tumor marker of oral cavity and play an important role in the treatment of oral tumor. Methods the overexpression and knockdown vector of SALL2 gene was constructed and introduced into oral cancer cell line SCC-3 to detect the expression of SALL2 gene in oral tumor cell line. The changes of cell proliferation and migration were observed by MTT assay and cell scratch assay, and the expression of SALL2 m RNA was detected by QRT-PCR method in 15 groups of oral tumor tissues and adjacent tissues. Result 1. The plasmid containing the target gene was designed and synthesized, and the plasmid was transferred into oral cancer cells by lentivirus packaging. By this method, stable over-expression or gene knockout of oral tumor cell line could be constructed. The results of scratch test showed that overexpression of SALL2 gene could inhibit migration of oral tumor cells, while knockout of SALL2 gene could enhance migration of oral tumor cells. The overexpression of SALL2 gene in SCC-3 cell lines was detected by MTT assay, which could inhibit the proliferation of oral cancer cells. However, knockout of SALL2 gene could enhance the proliferation of oral tumor cells. 4. The expression level of SALL2 m RNA in 15 groups was detected by QRT-PCR. The results showed that the expression of SALL2 m RNA in oral tumor tissues was decreased compared with that in normal tissues. Conclusion 1.SALL2 is involved in the occurrence and development of oral tumor and regulates the cell cycle of oral tumor through certain mechanism. Inhibiting the migration and growth of tumor cells. 2. SALL2 can be used as a potential tumor molecular marker for oral cancer to determine the biological behavior of tumor cells and to monitor tumor metastasis. 3. The regulatory mechanism of SALL2 expression in oral tumor tissues, the biological function and the specific mechanism of inhibiting tumor metastasis have not been elucidated. This study provides a theoretical basis for further study of the mechanism of SALL2 in oral tumor.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R739.8

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