AC、PKA基因水平表达变化对心气虚型冠心病的相关性研究

发布时间：2018-05-24 07:27

本文选题：益气活血方 + 心气虚　；参考：《时珍国医国药》2017年11期

【摘要】：目的探讨AC、PKA基因水平表达变化与心气虚型冠心病发病的相关性,以阐述心气虚型冠心病发病的微观机制。方法采用"冠状动脉结扎加注射左旋硝基精氨酸"法制备心气虚型冠心病大鼠模型,并将大鼠分为空白组、模型组、西药盐酸地尔硫卓组(3mg/kg)、益气活血方低、高、中剂量中药组(14.4,43.2,28.8g/kg),每组8只。造模成功后西药组用盐酸地尔硫卓干预,中药组用不同剂量的益气活血方干预,于造模45天后取心肌组织用PCR技术检测AC、PKA含量以观察基因水平表达变化。结果与模型组相比,中药组AC、PKA浓度均明显降低(P0.01);与西药组比较高剂量中药组AC、PKA浓度均明显下降(P0.01)。结论 AC、PKA基因水平表达变化与心气虚型冠心病存在相关性,AC、PKA基因水平表达变化在一定程度上揭示了心气虚型冠心病发病的微观机制。
[Abstract]:Objective to investigate the relationship between the expression of PKA gene in ACU and the pathogenesis of coronary heart disease (CHD) with deficiency of heart qi, and to elucidate the microcosmic mechanism of coronary heart disease (CHD) with deficiency of heart qi. Methods Coronary artery ligation and injection of L-nitroarginine were used to establish the rat model of coronary heart disease with deficiency of heart qi. The rats were divided into blank group, model group, diltiazem hydrochloride group (3 mg / kg), Yiqi Huoxue prescription low and high. Middle dose Chinese medicine group (n = 8, n = 8). After the model was successfully established, the western medicine group was treated with diltiazem hydrochloride, and the traditional Chinese medicine group with different doses of Yiqi Huoxue prescription. After 45 days of model making, myocardial tissue was taken to detect the content of ACK A by PCR technique to observe the change of gene level. Results compared with the model group, the concentration of ACP-PKA in the traditional Chinese medicine group was significantly lower than that in the western medicine group, and that in the high dose Chinese medicine group was significantly lower than that in the western medicine group. Conclusion there is a correlation between the expression of PKA gene and the level of PKA gene in patients with deficiency of heart qi. The changes of PKA gene level in patients with heart qi deficiency may reveal the microcosmic mechanism of coronary heart disease with deficiency of heart qi to a certain extent.
【作者单位】：山东中医药大学中医学院;
【基金】：国家自然科学基金(81273625)
【分类号】：R259

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