香蕉花芽分化期抗枯萎病相关基因表达分析

发布时间：2018-05-25 18:53

  本文选题：香蕉枯萎病 + 花芽分化期　； 参考：《生物技术通报》2017年07期

【摘要】：通过对‘巴西蕉’和‘宝岛蕉’花芽分化期响应枯萎病菌4号生理小种(Fusarium oxysporum f.sp.cubense race 4,Foc4)侵染的转录组进行分析,获得了大量差异表达基因。本研究以‘巴西蕉’和‘宝岛蕉’花芽分化期根部组织为材料,采用实时荧光定量PCR(q PCR)方法测定了10个与特定抗病机制相关基因的表达变化。结果表明,10个基因在‘巴西蕉’和‘宝岛蕉’花芽分化过程中均有表达,但其表达量存在差异。与对照‘巴西蕉’相比,除laccase-4(A)基因在‘宝岛蕉’中的表达量明显下调外,laccase-4(B)、periplasmic beta-glucosidase precursor、glutathione-S-transferase Cla47、class Ⅲ peroxidase 136 precursor、peroxidase N、similar to T-phylloplanin、snakin-1、putative chitinase及cellulose synthase catalytic subunit 12等9个基因的表达量均上调,其中peroxidase N基因的表达量呈极显著上调,相对表达量约为‘巴西蕉’的1 682倍。10个差异表达基因可能参与‘宝岛蕉’花芽分化期对病原菌的防卫反应,为进一步探讨‘宝岛蕉‘复杂的抗病防御反应分子机理奠定基础。
[Abstract]:A large number of differentially expressed genes were obtained by analyzing the transcriptome of Fusarium oxysporum f.sp.cubense race 4 (Foc4), a physiological species of Fusarium oxysporum f.sp.cubense race 4, in response to the floral bud differentiation of 'Canna brasiliensis' and 'Baodao banana'. In this study, the expression changes of 10 genes related to specific disease resistance mechanism were determined by real-time fluorescence quantitative PCR(q method using the root tissue of 'Canna bassiana' and 'Baodao banana' during flower bud differentiation. The results showed that all of the 10 genes were expressed in the process of flower bud differentiation, but there were differences in their expression levels. Compared with the control, the expression of laccase-4) gene was significantly down-regulated in 'Baodao banana', except that the expression of laccase-4 beta-glucosidase precursorsortium-glutathione-S-transferase Cla47 class 鈪，

本文编号：1934288