转基因抗虫棉对根际土壤Bt蛋白残留和养分含量的影响

发布时间：2018-05-28 00:46

本文选题：转基因抗虫棉 + Bt蛋白　；参考：《江苏农业科学》2017年06期

【摘要】：以转基因抗虫棉SGK321及其亲本常规棉石远321为研究对象,采用ELISA试剂盒法检测其在不同生育期根际土壤的Bt蛋白残留量,同时比较根际土壤速效养分含量的变化。结果表明:转基因抗虫棉SGK321根际土壤Bt蛋白残留量在整个生长期呈先上升后下降的趋势,且在各生育期均与亲本差异显著,其中蕾期转基因较亲本高996.09%。在整个生长期,2种棉花根际土壤各养分含量的变化不同,二者根际土壤硝态氮含量均呈下降趋势,仅在苗期、花铃期差异显著;转基因抗虫棉SGK321根际土壤中铵态氮含量呈下降趋势,在蕾期、花铃期与亲本差异显著;其速效磷含量呈先下降后上升趋势,亲本常规棉呈上升趋势,其中苗期转基因较亲本低40.13%。研究发现,转基因抗虫棉SGK321根际土壤Bt蛋白残留量和养分含量主要受转基因棉花种植及生育期的影响。
[Abstract]:The BT protein residues in rhizosphere soil of transgenic insect-resistant cotton (SGK321) and its parent cotton Shiyuan 321 were detected by ELISA kit method, and the changes of available nutrients in rhizosphere soil were compared. The results showed that BT protein residues in SGK321 rhizosphere soil of transgenic insect-resistant cotton increased first and then decreased during the whole growing period, and there were significant differences between transgenic cotton and their parents at each growth stage, among which the transgenic plants at bud stage were 996.09% higher than their parents in bud stage. The contents of nitrate in rhizosphere soil of two cotton varieties were different in the whole growing period, and the nitrate content in rhizosphere soil decreased, only in seedling stage, the difference was significant at flowering and boll stage. The content of ammonium nitrogen in the rhizosphere soil of transgenic insect-resistant cotton (SGK321) showed a decreasing trend, and there was a significant difference between the flower and boll stage in bud stage, and the content of available phosphorus in transgenic cotton decreased first and then increased, and the content of available phosphorus increased in parent conventional cotton, and 40.13% lower than that of parent in seedling stage. It was found that BT protein residue and nutrient content in SGK321 rhizosphere soil of transgenic cotton were mainly affected by planting and growing period of transgenic cotton.
【作者单位】：海南大学环境与植物保护学院;中国环境科学研究院;
【基金】：环保公益性行业科研专项(编号:201509044)
【分类号】：S562

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