基于玛氏骨条藻转录组的细胞死亡相关功能基因解析

发布时间：2018-05-29 11:43

本文选题：玛氏骨条藻 + 转录组分析　；参考：《中国海洋大学学报(自然科学版)》2017年04期

【摘要】：本研究以玛氏骨条藻(Skeletonema marinoi)转录组信息为基础,发现玛氏骨条藻中存在26个与细胞死亡相关的酶以及37个编码这些酶的基因。这些编码基因的序列比对结果表明,其与假微型海链藻(Thalassiosira pseudonana)和大洋海链藻(Thalassiosira oceanica)的同源基因具有较高的一致性。对这些样品进行数字基因表达谱的差异基因分析,获得了不同生长时期时与细胞死亡过程有关的编码基因的差异表达数据。结果发现,在稳定期和衰亡期,与抗氧化胁迫作用相关的关键酶(如过氧化物酶5、铜锌超氧化物歧化酶)、与RNA的加工与降解过程有关的功能基因(如多核糖核苷酸核苷转移酶、5’~3’核糖核酸外切酶2)以及细胞死亡特异蛋白的基因表达量显著高于指数期。本论文主要是通过对转录组数据进行分析得到基因转录组水平差异表达结果,为蛋白水平研究提供一种可能性变化趋势和潜在数据依据,同时为以后研究提供数据基础及研究方向。本研究也为进一步探讨硅藻的细胞死亡机制奠定了基础,也为深入理解赤潮的消亡过程提供了新的视角。
[Abstract]:Based on the transcriptional information of Skeletonema marinoi, 26 enzymes associated with cell death and 37 genes encoding these enzymes were found in this study. The sequence alignment results of these coding genes indicated that they were highly consistent with the homologous genes of Thalassiosira pseudonanaand Thalassiosira oceanica). The differentially expressed data of coding genes related to the process of cell death at different growth stages were obtained by the differential gene analysis of the digital gene expression profiles of these samples. And it turns out, in the stable period and in the decay phase, Key enzymes related to antioxidant stress (such as peroxidase 5, copper and zinc superoxide dismutase), functional genes related to the processing and degradation of RNA (e.g., polyribonucleotide ribonucleoside transferase 5 'ribonucleic acid) The gene expression of exonuclease 2) and cell death specific protein was significantly higher than that of index phase. In this paper, the results of differentially expressed gene transcriptome are obtained by analyzing the transcriptional data, which provides a possible change trend and potential data basis for protein level research. At the same time, it provides data basis and research direction for future research. This study also laid a foundation for the further study of diatom cell death mechanism and provided a new perspective for further understanding the extinction process of red tide.
【作者单位】：中国海洋大学海洋生命学院;海洋环境与生态教育部重点实验室;海洋国家实验室海洋生态与环境科学功能实验室;海洋化学理论与工程技术教育部重点实验室;
【基金】：国家自然科学基金项目(41521064) 海洋公益性行业科研专项(201205031) 山东省自然科学基金项目(ZR2014DM007)资助~~
【分类号】：Q943.2

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