基于PAL基因序列的地方苦荞品种遗传多样性分析

发布时间：2018-05-29 17:23

本文选题：苦荞 + PAL序列　；参考：《植物遗传资源学报》2017年03期

【摘要】：苦荞在中国具有广泛的栽培种植地区,长时间演化形成了丰富的遗传多样性。为了研究和利用苦荞资源,以国内北方苦荞产区(内蒙古、青海、陕西、山西、甘肃)、西南苦荞产区(西藏、四川、贵州、云南)、国内其他地方品种(江西、安徽、湖北、湖南、广西)及国外品种(尼泊尔)共计67份苦荞材料为研究对象,PCR扩增其PAL基因并测序。在此基础上分析苦荞的遗传多样性,并采用NJ法(neighbor-joining)对67份苦荞材料构建系统进化树。结果表明,供试的67份苦荞材料的PAL基因序列长度为2011 bp,其中,变异位点为160个,占序列总长度的7.9%,简约信息位点为33个,占序列总长度的1.64%,突变的类型主要是碱基的转换与颠换,高变异位点均集中在外显子2的N端。不同来源的苦荞材料间的遗传距离分布于0.002~0.016之间,来源于中国四川组的苦荞材料种内平均遗传距离最大(0.016),中国内蒙古组的最小(0.002)。中国四川地区的材料与其他地区来源的材料间的遗传距离位于0.010~0.016之间,而其他地区间的遗传距离为0.004~0.013。67份苦荞材料的平均π值和θ值分别为0.0034和0.0143。其中,中国四川材料的π值为0.0148。基于PAL基因序列构建的NJ进化树中,67份苦荞材料分为7个类群,分类与地理来源无关。仅中国西藏来源的5份材料聚集为一类,说明PAL基因序列较为稳定,多数材料间变化差异较小。中国四川地区的苦荞材料具有丰富的遗传多样性,中国西藏地区的某一材料中有较多的SNP位点,推测中国西藏的部分材料可能存在突变的热点区,预示着PAL基因新的突变位点区域。
[Abstract]:Tartary buckwheat (Tartary buckwheat) has a wide range of cultivated areas in China and has evolved into a rich genetic diversity over a long period of time. In order to study and utilize Tartary buckwheat resources in northern China (Inner Mongolia, Qinghai, Shaanxi, Shanxi, Gansu, Southwest China, Tibet, Sichuan, Guizhou, Yunnan, China), other local varieties in China (Jiangxi, Anhui, Hubei, Hubei) were studied. A total of 67 Tartary buckwheat materials from Hunan, Guangxi and Nepal were used to amplify and sequence their PAL genes. The genetic diversity of Tartary buckwheat was analyzed and the phylogenetic tree of 67 Tartary buckwheat materials was constructed by NJ method neighbor-joining. The results showed that the length of PAL gene of 67 Tartary buckwheat was 2011 BP, among them, the variation sites were 160, which accounted for 7.9% of the total sequence length, and 33 simple information sites. The mutation type was mainly base transformation and transversion, and the high mutation sites were concentrated in the N terminal of exon 2. The genetic distance of Tartary buckwheat from different sources ranged from 0.002 to 0.016. The average genetic distance of Tartary buckwheat from Sichuan group in China was the largest (0.016%), and that of Inner Mongolia group from China was 0.002%. The genetic distance between the materials from Sichuan region of China and the materials from other regions was 0.010 ~ 0.016, while the genetic distance between other regions was 0.004 ~ 0.013.67, the average 蟺 value and 胃 value of Tartary buckwheat were 0.0034 and 0.0143, respectively. The 蟺 value of Sichuan material in China is 0.0148. 67 Tartary buckwheat materials from NJ evolutionary tree constructed based on PAL gene sequence were divided into 7 groups, and the classification was independent of geographical origin. Only 5 materials from Tibet in China were clustered into one group, indicating that the sequence of PAL gene was relatively stable, and the variation of most materials was relatively small. Tartary buckwheat materials in Sichuan region of China have abundant genetic diversity, and there are many SNP loci in a certain material in Tibet area of China. It is speculated that some materials in Tibet of China may have hot spots of mutation. This indicates a new mutation site region of PAL gene.
【作者单位】：山西大学生物技术研究所;中国农业科学院作物科学研究所;国际生物多样性中心东亚办事处;
【基金】：科技部科技支撑计划项目(2013BAD01B05-2) 农业部国家作物种质资源保护专项(2015NWB030-06)
【分类号】：Q943.2;S517

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