枳SnRK2.4和SnRK2.6转化柠檬及转基因植株多胺含量与抗性分析

发布时间：2018-05-30 02:25

  本文选题：枳 + PtrSnRK2.4　； 参考：《华中农业大学》2017年硕士论文

【摘要】：SnRK2(Sucrose non-fermenting 1-related protein kinase2,蔗糖非酵解型蛋白激酶2)是植物中特有的一类丝氨酸/苏氨酸蛋白激酶,在ABA信号途径及植物抵御胁迫中起着关键作用。多胺是直接参与调控植物抗逆性的信号分子,其合成受到ABA信号途径的调控。但是SnRK2在调控多胺合成中的作用尚不清楚。本研究克隆了枳(Poncirus trifoliata(L.)Raf.)两个SnRK2基因PtrSnRK2.4与PtrSnRK2.6,利用根癌农杆菌介导柠檬转化获得了转基因植株,并对PtrSnRK2.4柠檬转基因系多胺含量及抗性进行分析。主要研究结果如下:1、从枳中克隆出PtrSnRK2.4,全长1026 bp,编码341个氨基酸;PtrSnRK2.6,全长1092 bp,编码363个氨基酸。PtrSnRK2.4和PtrSnRK2.6分别属于GroupⅢ和GroupⅡ家族。2、利用根癌农杆菌介导转化柠檬上胚轴,共获得PtrSnRK2.4转基因柠檬24株,PtrSnRk2.6转基因柠檬8株。对PtrSnRK2.4转基因系与PtrSnRK2.6转基因系相关基因表达量检测,发现在转基因株系中ABF1、ABF2和ADC表达量均高于野生型,PtrSnRK2.6转基因株系中ABF4也上调表达。并且PtrSnRK2.4转基因植株中腐胺含量均高于野生型。3、对PtrSnRK2.4转基因植株抗性进行分析,脱水处理50 min后,野生型比转基因植株萎蔫更严重,与之一致的是前者相对失水率和电导率也高于后者。表明PtrSnRK2.4转基因植株比野生型表现出更高的抗旱能力。
[Abstract]:SnRK2(Sucrose non-fermenting 1-related protein kinase2 (sucrose non-fermentable protein kinase 2) is a unique serine / threonine protein kinase in plants, which plays a key role in ABA signaling pathway and plant resistance to stress. Polyamines are signaling molecules directly involved in the regulation of plant stress resistance, and their synthesis is regulated by the ABA signaling pathway. However, the role of SnRK2 in the regulation of polyamine synthesis is unclear. In this study, Poncirus trifoliata L. Raf. was cloned. Two SnRK2 genes, PtrSnRK2.4 and PtrSnRK 2.6, were transformed into lemon by Agrobacterium tumefaciens. The polyamines content and resistance of PtrSnRK2.4 lemon transgenic lines were analyzed. The main results were as follows: 1. PtrSnRK2.4 was cloned from trifoliate orange, with a total length of 1026 BP, encoding 341 amino acids PtrSnRK2.6. the total length of PtrSnRK2.6 was 1092 BP, and the encoding of 363-amino acid. PtrSnRK2.4 and PtrSnRK2.6 belonged to Group 鈪，

本文编号：1953506