转SERCA 2a基因对急性心房颤动模型心房肌细胞L型钙通道影响的研究

发布时间：2018-05-30 13:47

本文选题：心房颤动 + 转基因　；参考：《临床心血管病杂志》2017年05期

【摘要】：目的:研究AAV9-SERCA 2a基因转染对急性心房颤动(房颤)动物模型心房肌细胞上L型钙通道表达的影响。方法:采用60只新西兰大白兔为实验对象,采用心包注射法转染AAV9-SERCA 2a基因;将实验对象随机分为4组:房颤组、转荧光蛋白房颤组、转SERCA 2a基因房颤组和对照组。转染后建立急性房颤模型,采用膜片钳及荧光免疫组织化学法检测心房肌细胞上L型钙通道的电流变化及其L型钙通道蛋白表达的情况。结果:转SERCA 2a基因房颤组心房肌细胞上LVDCCa1c蛋白表达明显高于房颤组(P0.05),与对照组比较差异无统计学意义。转SERCA 2a基因房颤组心房肌细胞上L型钙通道电流密度高于房颤组,差异有统计学意义(P0.05),与对照组比较差异无统计学意义。结论:转SERCA 2a基因治疗可减少急性房颤模型心房肌细胞钙超载,减少房颤对L型钙通道电流密度的影响,对防止心脏电重构具有积极的意义。
[Abstract]:Aim: to study the effect of AAV9-SERCA 2a gene transfection on the expression of L-type calcium channel in atrial myocytes of acute atrial fibrillation (AF) animal model. Methods: sixty New Zealand white rabbits were used to transfect AAV9-SERCA 2a gene by pericardial injection. The subjects were randomly divided into 4 groups: atrial fibrillation group, fluorescent protein atrial fibrillation group, SERCA 2a gene atrial fibrillation group and control group. The acute atrial fibrillation model was established after transfection. The changes of L-type calcium channel current and the expression of L-type calcium channel protein in atrial myocytes were detected by patch clamp and fluorescence immunohistochemistry. Results: the expression of LVDCCa1c protein in atrial myocytes in SERCA 2a gene group was significantly higher than that in atrial fibrillation group (P 0.05). The L-type calcium channel current density in atrial myocytes in SERCA 2a gene group was significantly higher than that in atrial fibrillation group (P 0.05), but there was no significant difference compared with the control group. Conclusion: SERCA 2a gene therapy can reduce the calcium overload of atrial myocytes in acute atrial fibrillation model, reduce the effect of atrial fibrillation on L-type calcium channel current density, and have a positive significance in preventing cardiac electrical remodeling.
【作者单位】：新疆医科大学第一附属医院内科;新疆医科大学第一附属医院干部综合内科;
【分类号】：R-332;R541.75

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