淇河鲫肌肉生长抑制素基因的克隆与表达

发布时间：2018-05-31 14:10

本文选题：淇河鲫 + 肌肉生长抑制素　；参考：《水产学报》2017年01期

【摘要】：肌肉生长抑制素(MSTN)是动物肌肉发育和生长过程中的负调控因子。为了明确淇河鲫MSTN基因序列及其在肌肉生长发育中的调控功能,本研究利用RACE方法克隆淇河鲫MSTN全长c DNA序列,q RT-PCR分析其在不同组织和胚胎发育阶段的表达情况。结果显示,淇河鲫MSTN c DNA序列全长2094 bp(no.KC851952.1),包含86 bp 5′非编码区、880 bp 3′非编码区和1128 bp开放阅读框,编码375个氨基酸残基,前22个氨基酸残基为信号肽,34~256位氨基酸残基为TGF-β前肽域,281~375是TGF-β结构域,具蛋白酶水解位点RIRR和C端生物活性区的9个保守半胱氨酸残基。蛋白同源分析发现淇河鲫MSTN与鲤形目鱼类相似性较高,与哺乳动物和鸟类的相似性最低。系统进化分析显示,淇河鲫MSTN与鲫、鲤、溼等鲤科鱼类亲缘关系较近。实时定量PCR分析表明,淇河鲫MSTN基因在各个组织中均有表达,脑中表达量最高,其次为肌肉和肝脏,肠道表达量最低。淇河鲫胚胎发育的各阶段MSTN基因均有表达,受精卵表达量最高,其次为囊胚期,神经胚期表达量最低。推测MSTN与淇河鲫肌肉发育生长具有一定的相关性,可能参与"双背鲫"特征的形成。
[Abstract]:Muscle growth inhibitor (MSTN) is a negative regulatory factor during muscle development and growth. In order to identify the MSTN gene sequence of Qihe crucian carp and its regulatory function in muscle growth and development, we used RACE method to clone the full-length c DNA sequence of MSTN from Qihe crucian carp and analyze its expression in different tissues and embryonic development stages. The results showed that the MSTN c DNA sequence of Qihe crucian carp was 2094 BP no. KC851952.1, including 880bp 3'noncoding region of 880bp and 1128 BP open reading frame, encoding 375 amino acid residues. The first 22 amino acid residues were signal peptide motif 34,256 amino acid residues, which were TGF- 尾 propeptide domain (TGF- 尾) and TGF- 尾 domain, and 9 conserved cysteine residues with protease hydrolysis site (RIRR) and C-terminal biological active region. Protein homology analysis showed that the similarity between MSTN and Cyprinidae was higher than that with mammals and birds. Phylogenetic analysis showed that MSTN was closely related to Carassius Carpio, Cyprinus Carpio and Cyprinidae. Real-time quantitative PCR analysis showed that MSTN gene was expressed in all tissues of Qihe crucian carp, the highest in brain, the second in muscle and liver, and the lowest in intestinal tract. The expression of MSTN gene was the highest in fertilized eggs, followed by blastocyst stage, and the lowest in nerve embryo stage. It is speculated that MSTN has some correlation with muscle development and growth of Qihe crucian carp, which may be involved in the formation of the characteristics of "double back crucian carp".
【作者单位】：河南师范大学水产学院河南省水产动物养殖工程技术研究中心;周口科技职业学院医学系;
【基金】：国家自然科学基金(31402294) 河南省国际科技合作计划项目(152102410040) 河南省科技攻关计划项目(132102110135,1421022110145)~~
【分类号】：Q78;S917.4

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