L-茶氨酸对大鼠内脏组织抗氧化能力及相关基因表达的影响

发布时间：2018-05-31 16:18

本文选题：L-茶氨酸 + 抗氧化能力　；参考：《食品科学》2017年03期

【摘要】：目的:通过对大鼠灌胃L-茶氨酸溶液,观察大鼠心脏、肝脏、脾脏和肾脏中抗氧化指标的变化,探究L-茶氨酸对大鼠心、肝、脾和肾抗氧化能力及其相关酶mRNA表达量的影响。方法:64只SD大鼠随机分为对照组、低剂量组、中剂量组和高剂量组4组(每组16只,雌雄各半),分别灌胃0、50、200、400 mg/(kg·d)L-茶氨酸。连续灌胃14 d后采集内脏组织,采用试剂盒检测样品中丙二醛(malondialdehyde,MDA)与一氧化氮(nitric oxide,NO)的含量,以及超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GPX)、过氧化氢酶(catalase,CAT)和一氧化氮合成酶(nitric oxide synthase,NOS)的活性,并采用实时荧光定量聚合酶链反应(real-time fluorescence quantitative polymerase chain reaction,RT-q PCR)法测定相应抗氧化酶的mRNA表达量。结果:L-茶氨酸灌胃影响大鼠心脏组织的NO含量,以及SOD、CAT与NOS活力,其中高剂量组大鼠心脏组织中NO含量最低,SOD与CAT活力最大;同时影响大鼠肾脏中NO的含量,高剂量组最低;但对除上述指标外的肝、脾、肾、心的MDA与NO含量,以及SOD、CAT、GPX与NOS活力无显著影响。低剂量L-茶氨酸灌胃显著抑制肝脏中SOD1 mRNA的表达和脾脏中SOD1、SOD3、GPX1 mRNA的表达;高剂量L-茶氨酸则上调脾脏SOD2 mRNA的表达;同时各剂量组心脏组织中的SOD1、SOD2、SOD3、GPX1 mRNA的表达量都显著增加。结论:L-茶氨酸灌胃增强了心脏的抗氧化能力,其机制可能是通过上调了大鼠心脏中SOD2基因的mRNA的表达,从而增加心脏中SOD的活性,起到抗氧化的保护作用。同时,L-茶氨酸灌胃对大鼠内脏抗氧化功能的影响存在性别差异。
[Abstract]:Aim: to investigate the effects of L-theanine on antioxidant activity and mRNA expression in rat heart, liver, spleen and kidney by intragastric administration of L-theanine. Methods Sixty-four Sprague-Dawley rats were randomly divided into four groups: control group, low dose group, middle dose group and high dose group. The contents of malondialdehyde (malondialdehyde) and nitric oxide (no) in the samples were detected by Elisa kit. The activities of superoxide dismutase (SOD), glutathione peroxidase (Glutathione peroxidase), catalase (catalase) and nitric oxide synthase (oxide synthase), Real-time fluorescence quantitative polymerase chain reaction- fluorescence quantitative polymerase chain RT-Q PCR was used to determine the mRNA expression of antioxidant enzymes. Results the content of no, the activity of cat and NOS in heart tissue of rats were significantly affected by WL-Theanine, and the activities of no and CAT were the lowest in the high dose group, and the lowest in the high dose group, at the same time, the content of no in the kidney was the lowest in the high dose group, and the lowest in the high dose group. But there was no significant effect on the contents of MDA and no in liver, spleen, kidney and heart, and the activities of NOS and GPX in liver, spleen, kidney and heart except for the above indexes. Low dose L-theanine significantly inhibited the expression of SOD1 mRNA in the liver and the expression of SOD3GPX1 mRNA in the spleen, high dose of L-theanine upregulated the expression of SOD2 mRNA in the spleen, and increased the expression of SOD1, SOD2, SOD3, GPX1 mRNA in heart tissue. ConclusionTwo L-theanine can enhance the antioxidant ability of the heart, and the mechanism may be that the expression of mRNA of SOD2 gene in rat heart can be upregulated, thereby increasing the activity of SOD in the heart and protecting the heart from oxidation. At the same time, the effect of L-theanine on visceral antioxidant function in rats was different in sex.
【作者单位】：湖南农业大学园艺园林学院湖南农业大学茶学教育部重点实验室湖南省植物功能成分利用协同创新中心;中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室湖南省畜禽健康养殖工程技术中心农业部中南动物营养与饲料科学观测试验站;永州职业技术学院药学系;
【基金】：中央驻湘科研机构技术创新发展专项(2013TF3006)
【分类号】：R151

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