Myostatin基因敲除对牛糖代谢的影响研究

发布时间：2018-05-31 21:24

本文选题：肌肉生长抑制素 + 生长性状　；参考：《内蒙古大学》2017年硕士论文

【摘要】：肌肉生长抑制素(myostatin,MSTN)作为骨骼肌发育的负调控因子,不仅与骨骼肌的发育有关,而且是哺乳动物机体内能量代谢重要调节者。葡萄糖作为机体代谢活动最重要的能量物质与MSTN基因存在关联性。目前关于MSTN蛋白功能的缺失对葡萄糖代谢的影响,主要集中在与胰岛素抵抗有关的信号通路以及葡萄糖转运体上。本试验以缺失6bp与115bpMSTN基因两种类型F1代牛为对象,研究了 MSTN敲除对F1代牛的生长发育、生理生化及糖代谢的影响。首先,对F1代犊牛自一月龄至十二月龄生长性状进行测量,确定了 MSTN基敲除对小牛生长发育的影响;其次,对犊牛进行血液MSTN蛋白含量的检测及液生化分析,比较了 MSTN敲除牛与对照组牛生理代谢物差异;最后,通过分析F1代牛细胞的葡萄糖转运、糖氧化、糖原合成及磷酸戊糖途径的底物及限速酶基因的表达情况,研究了 MSTN基因突变对于糖代谢的影响。结果表明:(1)与对照组相比,MSTN敲除牛血液中的MSTN蛋白含量极显著降低(P0.01)。(2)MSTN基因敲除可明显提升小牛的生长发育速度,MSTN短片段缺失组公牛生长指标均显著高于MSTN长片段缺失公牛与对照组公牛;MSTN敲除对于母牛生长的影响较公牛相比不显著。(3)血液生化结果显示,MSTN基因的敲除可以显著提升血液中碱性磷酸酶的含量,同时血液中的葡萄糖含量会显著降低,而谷氨酰胺转移酶、天冬氨酸氨基转移酶、丙氨酸氨基转移酶、血液淀粉酶、尿素氮、血磷、血钙、白蛋白、胆固醇、尿酸、肌酸激酶、肌酐、总胆红素、总蛋白、球蛋白等指标无明显差异。(4)MSTN敲除促进了糖摄取转运、糖氧化、磷酸戊糖途径相关的底物含量及相关限速酶的表达,同时又抑制了糖原合成。综上所述,MSTN基因敲除促进了牛的生长发育,影响与糖代谢有关的底物含量及限速酶基因的表达,提高了葡萄糖代谢,引起血糖浓度降低。
[Abstract]:As a negative regulator of skeletal muscle development, muscle growth inhibitor myostatinine (MSTN) is not only related to skeletal muscle development, but also an important regulator of energy metabolism in mammalian organism. Glucose, as the most important energy substance in metabolic activity, is associated with MSTN gene. At present, the effects of lack of MSTN protein function on glucose metabolism are mainly related to insulin resistance signaling pathway and glucose transporter. In this study, the effects of MSTN knockout on the growth, development, physiology, biochemistry and glucose metabolism of F1 cattle with deletion of 6bp and 115bpMSTN gene were studied. Firstly, the growth characteristics of F1 calves from one month to twelve months were measured, and the effects of MSTN knockout on calf growth and development were determined. Secondly, the blood MSTN protein content and liquid biochemical analysis were performed on calves. The differences of physiological metabolites between MSTN knockout cattle and control cattle were compared. Finally, the glucose transport, glucose oxidation, glycogen synthesis, substrate and rate-limiting enzyme gene expression of pentose phosphate pathway in F1 generation cattle were analyzed. The effect of MSTN gene mutation on glucose metabolism was studied. The results showed that compared with the control group, the content of MSTN protein in the blood of MSTN knockout cattle was significantly lower than that of the control group. The growth index of bull with short deletion of MSTN fragment was significantly higher than that of MSTN long fragment. The effect of MSTN knockout on the growth of cows was not significant compared with that of the control.) the results of blood biochemistry showed that the knockout of MSTN gene could significantly increase the content of alkaline phosphatase in the blood. At the same time, glucose levels in the blood decreased significantly, while glutamine transferase, aspartate aminotransferase, alanine aminotransferase, blood amylase, urea nitrogen, blood phosphorus, blood calcium, albumin, cholesterol, uric acid, creatine kinase, There was no significant difference in creatinine, total bilirubin, total protein and globulin. MSTN knockout promoted glucose uptake and transport, glucose oxidation, substrate content related to the pentose phosphate pathway and the expression of related rate-limiting enzymes, and inhibited glycogen synthesis at the same time. In conclusion, MSTN gene knockout promoted the growth and development of cattle, affected the substrate content related to glucose metabolism and the expression of rate-limiting enzyme gene, increased glucose metabolism, and resulted in the decrease of blood glucose concentration.
【学位授予单位】：内蒙古大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：Q78;S823

【相似文献】