糯玉米种质waxy基因的Eco-TILLING分析

发布时间：2018-06-01 06:32

  本文选题：糯玉米 + waxy基因　； 参考：《分子植物育种》2017年04期

【摘要】：本研究利用Eco-TILLING分析技术结合测序技术,对糯玉米种质材料的waxy基因序列变异进行分析。结果显示,在waxy基因从第9外显子到第14外显子总长为1 752 bp的序列中共发现72个突变位点;我们将156份糯玉米材料划分为13个单倍型,发现不同来源糯玉米种质糯质表现型的遗传基础表现出较大差异,在DNA序列上表现为SNP、InDel等不同类型的突变。对waxy基因序列进行进化分析表明,中国不同生态区糯玉米waxy基因序列具有不同的进化来源和进化方向,主要分为种群Ⅰ、种群Ⅱ两个大的种群,其中种群Ⅱ为中国西南地区特有的糯质玉米类型。研究探索了中国糯玉米种质遗传机制,发掘了有育种价值的有利等位基因资源,为糯玉米育种提供了理论依据和技术支持。
[Abstract]:In this study, Eco-TILLING analysis and sequencing were used to analyze the variation of waxy gene in waxy maize germplasm. The results showed that 72 mutation sites were found in the total length of 1 752bp from exon 9 to exon 14 of waxy gene, and we divided 156 waxy maize materials into 13 haplotypes. It was found that the genetic basis of waxy phenotypes of waxy maize germplasm from different sources was different, and the DNA sequence showed different types of mutations such as SNPN InDel. The evolutionary analysis of waxy gene sequence showed that the waxy gene sequence of waxy maize in different ecological regions of China had different evolutionary sources and evolutionary directions, mainly divided into two large populations: population 鈪，

本文编号：1963202