ALDH2基因和其他脂质代谢基因在麻风患者外周血中的表达

发布时间：2018-06-02 10:50

本文选题：乙醛脱氢酶 + 脂质代谢　；参考：《中国皮肤性病学杂志》2017年07期

【摘要】：目的研究乙醛脱氢酶2(aldehyde dehydrogenase 2,ALDH2)基因和脂质代谢与麻风的相关性。方法入选27例麻风病例和18例健康对照,转录组测序的方法比较了麻风病例皮损和健康对照中22个脂质代谢相关基因mRNA表达水平的差异。q PCR的方法检测了18例麻风患者和26例健康对照的外周血中ALDH2基因mRNA的表达水平。酶联免疫吸附测定(ELISA)的方法检测27例麻风患者和18例健康对照血清中ALDH2的浓度。结果麻风病例组和对照组皮损中22种脂质代谢相关基因表达差异有统计学意义(P0.05);麻风病例外周血中ALDH2的mRNA水平与健康对照差异无统计学意义(P=0.50);麻风病例血清中ALDH2浓度低于对照组,差异有统计学意义(P0.01)。结论麻风病例的脂质代谢相关基因与健康对照相比存在显著差异。麻风病例血清中乙醛脱氢酶2浓度显著低于健康对照,ALDH2可能通过调节脂质代谢影响麻风分枝杆菌对宿主的感染和麻风的发病。
[Abstract]:Objective to study the relationship between aldehyde dehydrogenase (2(aldehyde dehydrogenase _ 2) ALDH _ 2) gene, lipid metabolism and leprosy. Methods 27 cases of leprosy and 18 cases of healthy control were selected. The expression of ALDH2 gene mRNA in peripheral blood of 18 leprosy patients and 26 healthy controls was detected by the method of transcriptome sequencing. The difference of mRNA expression levels of 22 lipid metabolism-related genes between leprosy patients and healthy controls was compared. Serum ALDH2 levels were measured by Elisa in 27 leprosy patients and 18 healthy controls. Results there were significant differences in the expression of 22 lipid metabolism-related genes between the leprosy group and the control group (P 0.05), the mRNA level in peripheral blood of leprosy patients was not significantly different from that in the healthy control group, and the ALDH2 level in the serum of leprosy patients was not significantly different from that of the healthy controls. The concentration was lower than the control group. The difference was statistically significant (P 0.01). Conclusion there are significant differences in lipid metabolism related genes between leprosy patients and healthy controls. The concentration of acetaldehyde dehydrogenase 2 in serum of leprosy patients was significantly lower than that of healthy controls. ALDH2 may influence the infection of Mycobacterium leprosy on host and the pathogenesis of leprosy by regulating lipid metabolism.
【作者单位】：滨州医学院;山东省皮肤病性病防治研究所;
【基金】：山东省自然基金三院联合基金(ZR2014YL037)
【分类号】：R755

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