早期康复介入对脑损伤幼鼠的行为学及其体内降钙素基因相关肽表达的影响

发布时间：2018-06-02 12:42

本文选题：康复 + 缺血缺氧性脑损伤　；参考：《中国康复医学杂志》2017年10期

【摘要】：目的:研究早期康复治疗对缺血缺氧性脑损伤幼鼠的行为学及其体内降钙素基因相关肽表达的影响。方法:将40只15日龄SD大鼠平均分为4组,每组10只,分别为:脑损伤模型组:结扎大鼠左颈总动脉并置于含8%氧气和92%氮气的缺氧环境中,以此作为脑损伤模型;康复治疗组:造模后连续两周给予触摸(20 min/d)、丰富环境刺激(30 min/d)和平衡木训练(30min/d);假手术组:行左侧颈总动脉分离术后不作结扎及缺氧处理;对照组:不做处理。康复治疗两周后对各组大鼠进行平衡木实验、步伐失误实验和Morris水迷宫实验,并测定血浆、脑组织和脊髓中降钙素基因相关肽(CGRP)的含量。结果:与对照组相比,康复治疗组大鼠的平衡木实验评分增加1.6(P0.01),步伐失误率增加8.07%(P0.01),Morris水迷宫实验中的逃避潜伏期增加18.1s(P0.01),2min内跨越原平台的次数减少6次(P0.01),血浆、脑组织和脊髓中CGRP含量分别增加18.94pg/ml、103.38pg/ml和197.26pg/ml(P0.01);与脑损伤模型组相比,康复治疗组大鼠的平衡木实验得分减少1.8(P0.01),步伐失误率减少15.59%(P0.01),Morris水迷宫实验中的逃避潜伏期减少10.5s(P0.05),2min内跨越原平台的次数增加4次(P0.05),血浆、脑组织和脊髓中CGRP含量分别减少13.91pg/ml、86.57pg/ml和120.16pg/ml(P0.05)。结论:早期康复介入两周对脑损伤幼鼠的平衡能力、协调能力、学习记忆能力有明显改善,并能增加血浆、脑组织和脊髓中CGRP的含量,但未能恢复到正常水平。
[Abstract]:Objective: To study the effect of early rehabilitation on the behavior and the expression of calcitonin gene-related peptide in the young rats with ischemic hypoxic brain injury. Methods: 40 15 day old SD rats were divided into 4 groups, with 10 rats in each group, respectively, in the brain injury model group, ligation of the left cervical total vein in rats and in a hypoxic environment containing 8% oxygen and 92% nitrogen. This was used as a model of brain injury; the rehabilitation treatment group was given a touch (20 min/d) after two weeks of modeling, rich environmental stimulation (30 min/d) and balance wood training (30min/d); the sham operation group had no ligature and anoxia treatment after the left common carotid artery separation; the control group was not treated. After two weeks of rehabilitation treatment, the balance wood experiment of rats in each group was carried out. The content of calcitonin gene related peptide (CGRP) in plasma, brain tissue and spinal cord was measured by the felling error experiment and Morris water maze test. Results: compared with the control group, the balance wood test score of the rehabilitation group increased by 1.6 (P0.01), the rate of step error increased by 8.07% (P0.01), and the escape latency in the Morris water maze experiment increased by 18.1s (P0.01). The number of 2min across the original platform decreased by 6 times (P0.01), the CGRP content in plasma, brain tissue and spinal cord increased by 18.94pg/ml, 103.38pg/ml and 197.26pg/ml (P0.01). Compared with the brain damage model group, the score of the balance wood experiment in the rehabilitation group decreased by 1.8 (P0.01), the rate of step error decreased by 15.59% (P0.01), and the escape in the Morris water maze experiment The avoidance of latent period was 10.5s (P0.05), and the number of 2min across the original platform increased by 4 times (P0.05). The content of CGRP in plasma, brain tissue and spinal cord decreased 13.91pg/ml, 86.57pg/ml and 120.16pg/ml (P0.05). Conclusion: early rehabilitation intervention for two weeks can improve the balance ability, coordination ability, learning and memory ability of the young rats with brain injury, and can increase the ability of learning and memory. The content of CGRP in plasma, brain tissue and spinal cord did not return to normal level.
【作者单位】：南昌大学第一附属医院;
【基金】：江西省教育厅科学技术研究项目(14013)
【分类号】：R743.3

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