湖羊和巴什拜羊FSHR基因多态性分析

发布时间：2018-06-03 03:53

本文选题：湖羊 + 巴什拜羊　；参考：《畜牧与兽医》2017年05期

【摘要】：本文旨在了解绵羊(湖羊和巴什拜羊)促卵泡激素受体(FSHR)基因3'-UTR特征,分析高、低繁殖力绵羊品种FSHR基因3'-UTR突变位点多态性差异,并探索其机制。研究获得788 bp的湖羊和巴什拜羊FSHR基因3'-UTR序列,两者一致性为98.17%,且均含有加尾信号、保守的ARE元件和多个miRNA结合位点;池DNA测序在FSHR基因终止密码子后325nt处发现一个碱基T插入/缺失,命名为*325del T;多态性分析发现绵羊FSHR基因*325del T位点有3种基因型(TT型、T-型和--型),在湖羊群体中T为优势等位基因(频率为0.604),而在巴什拜羊群体中-为优势等位基因(频率为0.635);荧光素酶活性分析发现*325del T突变对绵羊FSHR转录活性无显著影响。发现FSHR基因*325del T的多态性可能与绵羊繁殖性能有关。
[Abstract]:The aim of this study is to understand the 3'-UTR characteristics of the follicle stimulating hormone receptor (FSHR) gene of sheep (Hu sheep and basbai sheep) and to analyze the polymorphism of 3'-UTR mutation at the FSHR gene of high and low fecundity sheep, and to explore its mechanism. The study obtained the FSHR based 3'-UTR sequence of 788 BP in Hu sheep and bahshibai sheep, and the consistency is 98.17%, and both contain tails. Signals, conservative ARE elements and multiple miRNA binding sites; pool DNA sequencing after FSHR gene termination codon 325nt found a base T insertion / deletion, named *325del T; polymorphism analysis found that the *325del T loci of the ovine FSHR gene were 3 genotypes (TT type, type and type) in the lake sheep population (frequency of 0.60) 4), in the basbai sheep population - the dominant allele (frequency 0.635), the luciferase activity analysis found that the *325del T mutation had no significant effect on the FSHR transcriptional activity of sheep. It was found that the polymorphism of the FSHR gene *325del T may be related to the reproductive performance of sheep.
【作者单位】：南京农业大学动物科技学院;新疆农业大学动物科学学院;
【基金】：江苏省自然科学基金(BK20141369) 江苏省农业科技自主创新资金项目[CX(15)1007]
【分类号】：S826

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