靶向Bmi-1基因RNA干扰对食管癌细胞增殖、侵袭及顺铂敏感性的作用

发布时间：2018-06-04 01:03

本文选题：Bmi- + siRNA　；参考：《实用医学杂志》2017年05期

【摘要】：目的:研究Bmi-1基因对食管鳞癌细胞增殖、侵袭、耐药的作用,并探讨其可能机制。方法:利用Bmi-1 siRNA转染食管癌细胞Eca9706,采用Westen-blot、CCK-8检测细胞增殖能力的改变,Transwell小室检测细胞侵袭能力的改变,并检测顺铂耐药性的改变。结果:Bmi-1 siRNA转染食管癌细胞Eca9706后,细胞增殖能力、侵袭能力均显著下降(P0.05),且顺铂耐药性显著下降。结论:Bmi-1基因具有促进食管癌细胞增殖、侵袭的作用,并促进顺铂耐药的作用。
[Abstract]:Aim: to study the effect of Bmi-1 gene on proliferation, invasion and drug resistance of esophageal squamous cell carcinoma cells. Methods: esophageal carcinoma cells Eca9706 were transfected with Bmi-1 siRNA. The changes of cell proliferation and cisplatin resistance were detected by Westen-blottCCK-8. Results the proliferation and invasion ability of human esophageal carcinoma cell line Eca9706 transfected with 10% Bmi-1 siRNA decreased significantly (P 0.05), and cisplatin resistance decreased significantly. Conclusion: Bmi-1 gene can promote the proliferation and invasion of esophageal cancer cells and promote cisplatin resistance.
【作者单位】：青海大学附属医院肿瘤内科;西宁市第二人民医院肿瘤内科;青海大学基础医学部;
【分类号】：R735.1

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