红肉苹果果实发育过程中花青苷含量变化及其合成相关基因表达分析

发布时间：2018-06-04 13:45

本文选题：红肉苹果 + 花青苷　；参考：《植物生理学报》2017年08期

【摘要】：本试验以白肉苹果(Malus pumila)‘澳洲青苹’为对照,研究了4个红肉苹果(M.sieversii f.neidzwetzkyana)品种(系)贵妃、‘红勋1号’、红勋5号、新疆1号在果实发育5个时期果肉花青苷含量,利用实时荧光定量PCR检测了花青苷合成相关结构基因和转录因子的表达水平。结果表明:(1)红肉苹果果肉中的花青苷含量在花后30 d最高,随后下降,红勋5号和新疆1号在花后120和150 d又有明显提高。(2)苹果花青苷合成的酶基因CHS、F3H和DFR在所有品种(系)果实发育的各个时期相对表达量相差不大;ANS基因在新疆1号和红勋5号的前3个发育时期表达量是‘澳洲青苹’的约9倍至约18倍,在后两个发育时期表达量降低;UFGT基因在‘红勋1号’、新疆1号和红勋5号果肉发育的各个时期中表达量均较高;MYB10转录因子在新疆1号和红勋5号盛花后30和60 d时的表达量为‘澳洲青苹’的约70倍至约90倍;TTG1、b HLH33在新疆1号和红勋5号中相对表达量高于b HLH3。(3)贵妃和‘红勋1号’MYB10启动子基因型为R1R6,红勋5号和新疆1号启动子基因型为R6R6,‘澳洲青苹’启动子基因型为R1R1。研究结果为红肉苹果分子育种提供理论参考。
[Abstract]:In this experiment, the contents of anthocyanin in the flesh of four red apple cultivars (lines), Gui 'Hongxun 1', Hongxun 5 and Xinjiang 1, were studied by using Malus pumilaer 'Australian green apple' as a control, and four red meat apple varieties (lines), M. sieversii f. Neidzwetzkyana, were used as control. The expression levels of structural genes and transcription factors related to anthocyanin synthesis were detected by real-time fluorescence quantitative PCR. The results showed that the content of anthocyanin in red apple pulp was the highest 30 days after anthesis and then decreased. Hongxun 5 and Xinjiang 1 increased significantly at 120 and 150 days after anthesis.) the relative expression of DFR and CHSN F3H and DFR in fruit development of all cultivars (lines) were similar in Xinjiang 1. The expression levels in the first three developmental stages of No.5 and Hongxun 5 were about 9 to 18 times of that of the Australian green apple. In the latter two developmental stages, the expression of UUFGT gene decreased in 'Hongxun 1'. The expression of MYB10 transcription factor in Xinjiang 1 and Hongxun 5 at 30 and 60 days after full bloom was 70 to 90 times higher than that of 'Qingpiao Australian'. The expression of MYB10 transcription factor was about 70 to 90 times higher than that of 'Qingping apple of Australia' at 30 and 60 days after the flowering of Xinjiang 1 and Hongxun 5. The relative expression levels in Xinjiang 1 and Hongxun 5 were higher than that in bHLH3. 3) the genotype of R1R6 in Guifei and 'Hongxun 1' MYB10 promoter was R1R6, and the genotype of R6R6 'Aussie' promoter in Hongxun 5 and Xinjiang 1 was R1R1. The results provide theoretical reference for molecular breeding of red meat apple.
【作者单位】：湖南农业大学园艺园林学院;青岛农业大学园艺学院青岛市园艺植物遗传改良与育种重点实验室;青岛农业大学生命科学学院山东省高校植物生物技术重点实验室;南京农业大学园艺学院;
【基金】：山东省良种产业化工程项目(620902) 青岛市民生科技计划项目(15-9-2-99-nsh) 国家现代苹果产业技术体系项目(CARS-28) 国家自然科学基金(31372032) 山东省优秀中青年科学家科研奖励基金(BS2014SW027)~~
【分类号】：S661.1

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