紫苏HPPR基因启动子的克隆及植物表达载体构建

发布时间：2018-06-05 11:13

本文选题：紫苏 + 迷迭香酸　；参考：《分子植物育种》2016年02期

【摘要】：以前期获得的紫苏HPPR基因的c DNA序列为模板,通过设计特异性引物和染色体步移的方法分离出HPPR基因启动子,全长2 216 bp,以此研究HPPR基因启动子的结构及功能特点。生物信息学分析表明,该序列中存在TATA-box和CAAT-box等典型的真核生物启动子基本元件,以及对茉莉酸甲酯、生长素、水杨酸和脱落酸等植物激素应答相关的顺式作用元件,另外也存在非生物胁迫顺式作用元件。将HPPR启动子部分缺失序列替代p BI121载体中的Ca MV35S启动子,构建了与GUS融合的启动子元件缺失表达载体。本试验的研究为该启动子在今后紫苏转基因定向改良提供了有用的候选资源。
[Abstract]:Using the cDNA sequence of HPPR gene obtained in the early stage as template, the promoter of HPPR gene was isolated by designing specific primers and chromosome step method. The promoter was 2216 BP in length. The structure and functional characteristics of HPPR gene promoter were studied. Bioinformatics analysis showed that there were typical eukaryotic promoter elements such as TATA-box and CAAT-box, and cis-acting elements related to phytohormone responses such as methyl jasmonate, auxin, salicylic acid and abscisic acid. There are also abiotic stress cis-acting elements. The partial deletion sequence of HPPR promoter was replaced by the Ca MV35S promoter in pBI121 vector, and the expression vector with Gus fusion promoter element deletion was constructed. This study provides a useful candidate resource for the future directed modification of perilla.
【作者单位】：天津科技大学食品工程与生物技术学院;
【基金】：国家863高技术研究发展计划(2007AA100401)资助
【分类号】：S567.219

【相似文献】