棉花BZR基因家族的全基因组鉴定及表达分析

发布时间：2018-06-05 17:25

  本文选题：棉花 + BZR基因　； 参考：《棉花学报》2017年05期

【摘要】：【目的】油菜素唑抗性因子(Brassinazole resistant transcription factor,BZR)是植物油菜素内酯(Brassinosteroid,BR)信号通路中的1类重要转录因子,通过调控相关基因的表达,参与植物生长发育过程。本研究旨在探索棉花中BZR基因的数量及其功能。【方法】利用生物信息学方法,对雷蒙德氏棉、亚洲棉和陆地棉中的BZR基因进行全基因组鉴定及表达模式分析。【结果】雷蒙德氏棉、亚洲棉和陆地棉中分别有7、7和14个BZR基因;通过序列特征分析和系统发育分析可将棉花BZR基因分为2组(a组和b组)。内含子/外显子结构分析发现:大部分BZR基因具有2个外显子,a组成员中均具有较长的内含子序列,b组成员的内含子序列较短,同组BZR成员具有相似的基因结构。基因复制分析表明:片段复制是棉花BZR基因家族扩增的主要方式,棉花BZR基因在进化过程中经历了严格的纯化选择。转录组数据分析表明:14个Gh BZR基因在陆地棉的7个组织中特异表达,大多数Gh BZR基因在茎、叶、花瓣和雌蕊中高表达;许多Gh BZR基因的表达受非生物胁迫诱导。【结论】推测Gh BZR基因可能参与棉花抗逆及纤维发育。本研究结果可为棉花BZR基因的生物学功能研究提供信息。
[Abstract]:[objective] Brassinazole resistant transcription factor (BZR) is an important transcriptional factor in the signal pathway of Brassinosteroido Brassinosteroidae, which is involved in plant growth and development by regulating the expression of related genes. The purpose of this study was to explore the number and function of BZR gene in cotton. The BZR gene in Asian cotton and upland cotton were identified and expressed in the whole genome. [results] there were 7 BZR genes and 14 BZR genes in Raymond's cotton, Asian cotton and upland cotton, respectively. Through sequence analysis and phylogenetic analysis, the cotton BZR gene can be divided into two groups: group A and group b. The analysis of intron / exon structure showed that most members of BZR gene had longer intron sequence and shorter intron sequence, and similar gene structure of the same BZR member. Gene replication analysis showed that fragment replication was the main way to amplify the BZR gene family in cotton, and the BZR gene in cotton underwent strict purification and selection during the evolution process. Transcriptome analysis showed that 14 Gh BZR genes were specifically expressed in 7 tissues of Upland cotton, and most of them were highly expressed in stems, leaves, petals and pistil. Many expression of Gh BZR gene was induced by abiotic stress. [conclusion] it is suggested that Gh BZR gene may be involved in stress resistance and fiber development of cotton. The results of this study may provide information for the study of the biological function of cotton BZR gene.
【作者单位】： 中国农业科学院棉花研究所/棉花生物学国家重点实验室;河南科技学院/现代生物育种河南省协同创新中心;
【基金】：国家自然科学基金(31671745) 国家重点研发计划(2016YFD0101401,2016YFD0100203)
【分类号】：S562
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本文编号：1982830