干扰Mig-6基因表达对非小细胞肺癌EGFR信号通路的影响

发布时间：2018-06-05 19:22

本文选题：丝裂原诱导因子- + 非小细胞肺癌　；参考：《东南大学学报(医学版)》2017年03期

【摘要】：目的:探讨干扰丝裂原诱导因子-6(Mig-6)基因表达对非小细胞肺癌表皮生长因子受体(EGFR)信号通路的影响。方法:A549、H1650细胞系均分为阴性对照组(si-NC组)和阳性实验组(si-Mig-6组),si-Mig-6组采用特异性小干扰RNA(siRNA)干扰Mig-6表达,si-NC组仅加入不含质粒的空载体,转染后采用MTT法检测细胞增殖能力,检测各组细胞侵袭能力和EGFR、ERK、AKT的蛋白及mRNA表达水平。结果:siRNA转染72 h后,si-Mig-6组Mig-6 mRNA及蛋白表达均显著低于si-NC组(P0.05),细胞增殖能力显著高于siNC组,细胞侵袭能力亦显著高于si-NC组(P0.05)。Mig-6对EGFR信号通路具有负向调控作用,干扰Mig-6表达,si-Mig-6组EGFR、细胞外调节蛋白激酶(ERK)、丝氨酸苏氨酸蛋白激酶(AKT)蛋白及mRNA表达均显著高于si-NC组(P0.05)。结论:干扰Mig-6表达能够增强非小细胞肺癌增殖、侵袭能力,其可能通过对EGFR通路的负向调控而实现这一生物学功能,Mig-6有望成为治疗非小细胞肺癌一个潜在基因靶点。
[Abstract]:Aim: to investigate the effect of interfering mitogen-inducible factor-6 (Mig-6) gene expression on EGFR signaling pathway in non-small cell lung cancer (NSCLC). Methods the cell lines were divided into negative control group (n = 10) and positive group (n = 6). The small interference RNA-Mig-6 group was used to interfere the expression of Mig-6. The empty vector without plasmid was added only in the control group. After transfection, the proliferation of the cells was detected by MTT method. The invasiveness and the protein and mRNA expression of EGFR ERKT were detected. Results 72 h after transfection, the expression of Mig-6 mRNA and protein in si-mig-6 group was significantly lower than that in si-NC group, and the cell proliferation ability was significantly higher than that in siNC group, and the cell invasion ability was significantly higher than that in si-NC group. Mig-6 had a negative regulatory effect on EGFR signaling pathway. The expression of Mig-6, extracellular regulated protein kinase (ERK), serine threonine protein kinase (Serine threonine protein kinase) and mRNA in Mig-6 group were significantly higher than those in si-NC group (P 0.05). Conclusion: interfering with the expression of Mig-6 can enhance the proliferation and invasion ability of non-small cell lung cancer, which may be a potential gene target for the treatment of non-small cell lung cancer through the negative regulation of EGFR pathway.
【作者单位】：莱芜市人民医院呼吸内科;
【分类号】：R734.2

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