钙反应性反式激活因子在肌萎缩侧索硬化症转基因鼠中的表达及意义

发布时间：2018-06-06 02:52

本文选题：钙反应性反式激活因子 + 肌萎缩侧索硬化症　；参考：《中国老年学杂志》2017年17期

【摘要】：目的探讨钙反应性反式激活因子(CREST)在肌萎缩侧索硬化症(ALS)转基因鼠脊髓及脑干组织中的表达变化及相关性作用。方法分别取出生后95 d、108 d、122 d(早、中、晚期)野生型与SOD1-G93A转基因鼠,通过RT-PCR、蛋白免疫印迹技术检测脊髓组织中CREST mRNA及蛋白水平表达变化,通过免疫荧光双标记技术检测脊髓及脑干组织中CREST的表达变化及与神经元及星形胶质细胞的共表达情况。结果 (1)与野生型鼠相比较,SOD1-G93A转基因鼠脊髓组织中CREST蛋白水平下调(P0.05),在mRNA水平变化并不明显。(2)免疫荧光双标检测显示,CREST主要表达于细胞核,与神经元有共表达,与星形胶质细胞不存在共表达;与野生型鼠比较,SOD1-G93A转基因鼠脊髓和脑干组织中CREST表达下调(P0.05)。结论 CREST的差异表达可能与ALS运动神经元的功能障碍有关。
[Abstract]:Objective to investigate the expression and correlation of calcium reactive transactivator (CREST) in spinal cord and brain stem of amyotrophic lateral sclerosis (ALS) transgenic rats. Methods the expression of CREST mRNA and protein in spinal cord tissue was detected by RT-PCR and Western blotting technique in wild type and SOD1-G93A transgenic mice of 122nd day (early, middle and late stage) after 95 days of birth, and the expression of CREST mRNA and protein in spinal cord tissue were detected by RT-PCR and Western blot. The expression of CREST in spinal cord and brain stem and co-expression with neurons and astrocytes were detected by immunofluorescence double labeling technique. Results compared with wild type mice, the level of CREST protein in the spinal cord of SOD1-G93A transgenic mice was down-regulated, but the change of mRNA level was not obvious. The double immunofluorescence double labeling assay showed that CREST was mainly expressed in the nucleus and coexpressed with neurons. The expression of CREST in spinal cord and brain stem tissues of SOD1-G93A transgenic mice was down-regulated as compared with that of wild type mice. Conclusion the differential expression of CREST may be related to the dysfunction of ALS motoneurons.
【作者单位】：潍坊医学院病理学教研室;潍坊医学院组织学与胚胎学教研室;潍坊医学院人体解剖学教研室;
【基金】：国家自然科学基金青年基金(81271413,81401066) 山东省科技发展计划项目(2012GSF11827) 山东省自然科学基金(ZR2012HQ021) 山东省教育厅课题(J12LK51,J13LK05) 山东省医药卫生科技发展计划项目(2013WS0279)
【分类号】：R744.8

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