C-myc基因表达及细胞凋亡在脂溢性角化病皮损中的意义

发布时间：2018-06-06 15:16

本文选题：脂溢性角化病 + 免疫组织化学　；参考：《中国现代医学杂志》2017年11期

【摘要】：目的观察脂溢性角化病(SK)中C-myc基因表达及细胞凋亡情况,探讨脂溢性角化病的发病机制。方法对66例脂溢性角化病皮损及10例正常皮肤组织采用免疫组织化学法检测C-myc基因表达及原位细胞凋亡检测(TUNEL)法检测细胞凋亡情况。结果两组性别、年龄差异均无统计学意义(P0.05);SK组皮损区C-myc基因阳性细胞数百分比(21.94±19.45)%与对照组(0%)相比增加(P0.05);66例SK皮损中有52例C-myc基因表达阳性,对照组中均无阳性表达,两组阳性率差异有统计学意义(P0.05);TUNEL凋亡检测中SK组的凋亡指数(53.01±31.97)与对照组(33.50±23.86)比较,差异无统计学意义(P0.05);Spearman相关性分析显示,C-myc基因在SK皮损中阳性细胞百分比与SK皮损中细胞凋亡指数差异无统计学意义(P0.05)。结论 C-myc基因参与脂溢性角化病的发生发展,脂溢性角化病皮损中细胞凋亡有所增加。该结果对研究其发病机制有一定的指导意义。
[Abstract]:Objective to investigate the expression of C-myc gene and apoptosis in seborrheic keratosis (SKK) and to explore the pathogenesis of seborrheic keratosis. Methods the expression of C-myc gene in 66 cases of seborrheic keratosis and 10 cases of normal skin were detected by immunohistochemical method and Tunel method. Results there was no significant difference in sex and age between the two groups. The percentage of C-myc gene positive cells in skin lesions of the two groups was 21.94 卤19.45%. Compared with the control group, 52 cases of SK lesions were positive for C-myc gene expression, and no positive expression was found in the control group. The difference of positive rate between the two groups was statistically significant. The apoptosis index of SK group (53.01 卤31.97) was significantly higher than that of control group (33.50 卤23.86). The results of Spearman correlation analysis showed that there was no significant difference between the percentage of positive cells of C-myc gene in SK lesions and apoptosis index in SK lesions. Conclusion C-myc gene is involved in the occurrence and development of seborrheic keratosis, and apoptosis is increased in seborrheic keratosis. The results have a certain guiding significance for the study of its pathogenesis.
【作者单位】：承德医学院附属医院;广州中医药大学;河北省沧州市中心医院;
【分类号】：R739.5

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