非小细胞肺癌171例患者EGFR基因突变分析

发布时间：2018-06-07 06:39

本文选题：肺恶性肿瘤 + EGFR基因　；参考：《广东医学》2017年13期

【摘要】：目的研究171例非小细胞肺癌(NSCLC)患者表皮生长因子受体(EGFR)基因突变状况,为肺癌的基因诊断和靶向治疗提供依据。方法 171例肺癌患者取新鲜病理组织标本,用厦门艾德核酸提取试剂提取体细胞DNA,采用扩增阻滞突变系统荧光PCR(ARMS-PCR)技术检测EGFR基因突变。结果在95例男性肺癌患者中,共有4例患者EGFR基因同时检测到存在2个位点突变。171例NSCLC病例中,EGFR基因野生型比率为48.57%(85/175),EGFR基因突变型比率为51.43%(90/175),突变型中21L858R点突变占24.00%(42/175),19Del突变占21.71%(38/175)。男女患者比较,EGFR基因野生型比率男性为57.58%(57/99),女性为36.84%(28/76),差异有统计学意义(P=0.007);EGFR基因19Del女性为28.95%(22/76),男性为16.16%(16/99),差异有统计学意义(P=0.042)。EGFR基因21L858R点突变女性为28.95%(22/76),男性为20.20%(20/99),差异无统计学意义(P=0.179)。结论 NSCLC患者EGFR基因突变率大约为50%左右,突变型以21L858R和19Del占大多数,突变型中女性显著多于男性。
[Abstract]:Objective to study the status of EGFR gene mutation in 171 patients with non-small cell lung cancer (NSCLC) and to provide evidence for gene diagnosis and targeted therapy of lung cancer. Methods somatic DNA was extracted from 171 patients with lung cancer by using Xiamen Eddard nucleic acid extraction reagent. The mutation of EGFR gene was detected by PCR PCR ARMS-PCR. Results in 95 male patients with lung cancer, 4 patients with EGFR gene were found to have two loci mutation. In 171 cases with NSCLC, the wild type ratio of EGFR gene was 48.57%. The mutation rate of EGFR gene was 51.4390 / 175%, and the 21L858R point mutation accounted for 24.0042 / 1755% 19Del mutation. The wild-type ratio of EGFR-gene in males and females was 57.58 / 57 / 99 and 36.8484 / 28 / 76, respectively. The difference was statistically significant (P < 0.01). The difference was statistically significant (P < 0.05). The difference was significant (P < 0.01). The difference was significant (P < 0.05). The 21L858R mutation of EGFR gene was 28.9595% 22 / 76% in women, and 20.20% 20 / 76% in women. The difference was not statistically significant (P 0.179 9). Conclusion the mutation rate of EGFR gene in patients with NSCLC is about 50%, 21L858R and 19Del are the majority of the mutations, and the number of females is significantly higher than that of males.
【作者单位】：广州市第一人民医院普通内科;广州市第一人民医院病理科;广州市第一人民医院心胸外科;
【分类号】：R734.2

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