青花菜锌指蛋白基因BoCCCH1的克隆和表达分析

发布时间：2018-06-07 08:56

本文选题：CCCH锌指蛋白 + 霜霉病　；参考：《核农学报》2016年03期

【摘要】：CCCH锌指蛋白广泛存在于真核生物中,在植物的生长、发育和逆境胁迫响应中起着重要作用。为获知青花菜CCCH转录因子基因的序列特征和表达特性,从青花菜叶片中克隆到1个CCCH基因,命名为Bo CCCH1,同时,利用RT-PCR方法研究了其在不同器官及霜霉菌侵染叶片中的表达模式。序列分析结果表明,Bo CCCH1的基因组全长为1 568 bp,包含1个长度为599 bp的内含子,2个长度分别为627 bp、342 bp的外显子,编码322个氨基酸,蛋白质的分子量与等电点分别为35 296.42 Da和8.47;Bo CCCH1有2个锌指结构,类型均为C-X8-C-X5-C-X3-H。系统发育分析结果表明,Bo CCCH1与其它植物的21条同源序列在进化树上分为6组,来自十字花科的CCCH与Bo CCCH1处于同一分支。基因表达结果表明,Bo CCCH1在叶、花茎、嫩角果、花蕾和花中表达,其中花茎和嫩角果的表达量最高,但在根中未检测到表达;在霜霉菌侵染下,叶片Bo CCCH1的表达量升高,到24 h和36 h时达到最大,推测Bo CCCH1与霜霉病抗性相关。本研究为进一步探明Bo CCCH1在霜霉病抗性反应中的功能奠定了基础。
[Abstract]:Zinc finger protein (CCCH) is widely found in eukaryotes and plays an important role in plant growth, development and stress response. In order to know the sequence and expression characteristics of CCCH transcription factor gene in broccoli, a CCCH gene named Bo CCCH1 was cloned from cauliflower leaves. The expression patterns of CCCH gene in different organs and infected leaves were studied by RT-PCR method. Sequence analysis showed that the genome of Bo CCCH1 was 1 568 BP in length, including one intron with 599 BP in length and two exons with a length of 627 BP, encoding 322 amino acids. The molecular weight and isoelectric point of the protein are 35 296.42 Da and 8. 47% Bo CCCH1, respectively, with two zinc-finger structures, all of which are C-X8-C-X5-C-X3-H. Phylogenetic analysis showed that the 21 homologous sequences of Bo CCCH1 and other plants were divided into 6 groups in the evolutionary tree, and CCCH from Cruciferae and Bo CCCH1 were in the same branch. The results of gene expression showed that Bo CCCH1 was expressed in leaves, stems, tender kernels, buds and flowers, among which the highest expression was found in stems and tender pods, but no expression was detected in roots. At 24 h and 36 h, the highest value was obtained, which suggested that Bo CCCH1 was associated with downy mildew resistance. This study laid a foundation for further exploring the function of Bo CCCH1 in downy mildew resistance response.
【作者单位】：台州学院生命科学学院/生态学浙江省重点学科;丽水学院生态学院;
【基金】：浙江省本科院校中青年学科带头人学术攀登项目(pd2013420) 浙江省自然科学基金项目(LY13C150003) 浙江省重点学科(浙江省台州学院生态学)开放课题(EKD2013-03)
【分类号】：S635.3

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