巨尾桉铜分子伴侣EuCCS基因的克隆及表达分析

发布时间：2018-06-07 14:26

本文选题：巨尾桉 + 铜分子伴侣　；参考：《华侨大学学报(自然科学版)》2017年03期

【摘要】：以巨尾桉为研究对象,克隆得到巨尾桉铜分子伴侣(CCS)基因(GenBank注册号为KJ755351.1),生物信息学分析表明:EuCCS蛋白定位于叶绿体,与龙眼同源基因的一致性达到99%.巨尾桉CCS的原核表达载体pET-EuCCS在大肠杆菌细胞内可以稳定表达,超氧化物歧化酶(SOD)活检测结果表明:转化菌株的SOD酶活性比对照菌株高,说明外源基因EuCCS具有生物学活性.利用荧光定量聚合酶链式反应(PCR)技术分析CCS在巨尾桉中的表达特性,结果表明:在植株生长旺盛的部位,CCS表达量较大,如植株幼叶,随植株叶片慢慢衰老,CCS的表达量也随之下降;在组培苗中,叶片的CCS表达量最大,其次是茎.
[Abstract]:Taking Eucalyptus grandis as the research object, the GenBank registration number of CCS gene was KJ755351.1. Bioinformatics analysis showed that the protein was located in chloroplast and the homologous gene was 99g. The prokaryotic expression vector pET-EuCCS of Eucalyptus grandis CCS was stably expressed in Escherichia coli cells, and the activity of superoxide dismutase (SOD) was detected. The results showed that the SOD activity of the transformed strain was higher than that of the control strain, indicating that the exogenous gene EuCCS had biological activity. Fluorescence quantitative polymerase chain reaction (PCR) technique was used to analyze the expression characteristics of CCS in Eucalyptus grandis. The expression of CCS decreased with the senescence of plant leaves, and the CCS expression was the highest in the plantlets in tissue culture, followed by the stems.
【作者单位】：华侨大学化工学院;
【基金】：国家自然科学基金资助项目(31300497)
【分类号】：Q943.2;S792.39

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