基于2b-RAD技术的家蚕BmNPV抗性关联基因的全基因组筛查

发布时间：2018-06-08 10:49

本文选题：家蚕 + 家蚕核型多角体病毒　；参考：《蚕业科学》2017年06期

【摘要】：不同家蚕品种对家蚕核型多角体病毒(Bm NPV)的抵抗性存在较大差异,家蚕抗性品系AN、野BN对Bm NPV侵染具有较强的抵抗性。用易感家蚕品系C108分别与AN和野BN杂交、回交,构建两个抗性品系的BC3M和BC4M分离群体,利用简化基因组测序技术2b-RAD(基于IIB型限制性内切酶的RAD)进行亲本和杂交分离群体的基因组测序,通过生物信息学方法筛选位于染色体上与抗性相关的多态性SNP标记,进行抗性品种的基因型分析。对3个亲本及2个抗性分离群体样本基因组DNA构建的2b-RAD标签文库进行Illumina测序,获得抗性亲本AN、野BN的多态性SNP标记11 919个和12 293个。对抗性分离群体全基因组染色体的Bm NPV抗性关联SNP标记指数(SNP-index)进行分析,结果表明亲本品系AN、野BN的Bm NPV抗性相关SNP标记分布在多个染色体上,其中AN的全基因组中抗性贡献率前5位是第5、10、27、23和4号染色体,野BN的全基因组中抗性贡献率前5位的是第4、27、15、18和6号染色体,有多个SNP-index高值位点与已知Bm NPV抗性相关基因的位置具有较高的一致性。推测AN、野BN两个品系的Bm NPV高抗性与抗性主基因和其他抗性基因的联合作用有关。
[Abstract]:The resistance of different silkworm varieties to Bombyx mori nuclear polyhedrosis virus (Bombyx mori Bm NPV) was different, and ANM NPV strain and wild BN strain had strong resistance to Bm NPV infection. The isolates of BC3M and BC4M were constructed by crossing susceptible silkworm strain C108 with an and wild BN, respectively. The simplified genomic sequencing technique 2b-RAD (based on IIB restriction endonuclease) was used to sequence the genomes of parents and hybrids, and the polymorphic SNP markers associated with resistance on chromosomes were screened by bioinformatics. Genotypic analysis of resistant varieties was carried out. The 2b-RAD tag library constructed from genomic DNA of three parents and two resistant segregated populations was sequenced by Illumina, and 11 919 and 12 293 polymorphic SNP markers of wild BN were obtained. The analysis of BmNPV resistance associated SNP marker index (SNP-index) showed that Bm NPV resistance-related SNP markers were distributed on multiple chromosomes. In the whole genome of an, the highest contribution rate of resistance was chromosome 5 (chromosome 5), and the contribution rate of resistance in the whole genome of wild BN was chromosome 427 (1518) and chromosome 6 (6). Multiple SNP-index high value loci were consistent with known Bm-NPV resistance related genes. It was inferred that the high resistance of Bm NPV in ANBN and wild BN strains was related to the combination of resistant major genes and other resistant genes.
【作者单位】：江苏科技大学生物技术学院;中国农业科学院蚕业研究所农业部蚕桑遗传改良重点实验室;
【基金】：现代农业产业技术体系建设专项(No.CARS-18)
【分类号】：S881.2

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