刺五加鲨烯合酶基因DNA和启动子的克隆及分析

发布时间：2018-06-08 17:12

本文选题：刺五加 + 鲨烯合酶基因　；参考：《分子植物育种》2017年04期

【摘要】：在已克隆出的刺五加鲨烯合酶基因(squalene synthase,SS)基因cDNA序列基础上,利用TAIL-PCR技术克隆SS的基因组DNA及启动子序列。克隆得到刺五加SS的DNA序列长8 244 bp,启动子序列长1 984 bp,转录起始位点位于起始密码子ATG上游568 bp处。基因包括13个外显子,12个内含子,其剪切符合GT-AG原则。启动子序列含有37个顺式作用元件,其中包括128个TATA-box、38个CAAT-box等核心调控元件,并且含有部分光响应元件、激素应答的元件等顺式调控元件。本研究首次克隆出刺五加SS基因组DNA及启动子序列,为后续研究SS基因表达的调控机制有很大帮助。
[Abstract]:Based on the cloned cDNA sequence of squalene synthase (SSSS) gene from Acanthopanax senticosus, the genomic DNA and promoter sequence of SS were cloned by TAIL-PCR. The DNA sequence of SS of Acanthopanax senticosus was 8 244 BP long, the promoter was 1 984 BP long, and the transcription initiation site was 568 BP upstream of the start codon ATG. The gene consists of 13 exons and 12 introns, and its cleavage conforms to the GT-AG principle. The promoter sequence contains 37 cis-acting elements, including 128 TATA-box, 38 CAAT-box, and some photo-responsive elements, hormone response elements and other cis-regulatory elements. In this study, the genomic DNA and promoter sequences of SS from Acanthopanax senticosus were cloned for the first time, which was helpful for further study on the regulation mechanism of SS gene expression.
【作者单位】：华北理工大学生命科学学院;华北理工大学药学院;
【基金】：国家自然科学基金项目(31570683) 河北省教育厅资助科研项目(QN2014102) 华北理工大学培育基金(SP201508);华北理工大学大学生创新创业训练计划项目(X2016046)共同资助河北省大学生创新创业训练计划(201610081046)
【分类号】：Q943.2;S567.19

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