葡萄风信子FLS1基因克隆及其表达与花色性状之间的关联性分析

发布时间：2018-06-09 02:26

本文选题：葡萄风信子 + 花色　；参考：《西北林学院学报》2017年01期

【摘要】：黄酮醇合成酶(flavonol synthase,FLS)是类黄酮合成分支路口重要的节点酶。FLS基因的表达不仅影响着黄酮醇合成,也影响着花青素苷积累和花色呈现。本研究采用PCR技术从葡萄风信子‘白丽人’中克隆到一条FLS基因(MaFLS1)。序列分析表明,MaFLS1cDNA全长1 152bp,编码383个氨基酸。同源比对及进化分析表明,MaFLS1属于2-酮戊二酸与Fe+2依赖型双加氧酶蛋白,具有典型的FLS蛋白功能域、DHQ底物特异结合位点、Fe2+和2-酮戊二酸绑定位点;MaFLS1与海枣、油棕的FLS同源性最高,可达74%~75%,与拟南芥、矮牵牛等模式植物亲缘关系较远。荧光定量PCR分析发现,MaFLS1基因在葡萄风信子中为非组织特异性表达模式,其根中表达量最高,鳞茎及花中的表达量其次,叶片中表达量最低;MaFLS1在3个不同花色的葡萄风信子品种5个不同花发育时期表达差异显著,在白色品种‘白丽人’花发育的早期(S1和S2)时期表达较高,粉色品种‘粉日出’和蓝色品种‘亚美尼亚’晚期(S3和S4)表达量最高。本研究为进一步探讨葡萄风信子FLS基因在花色呈现中的功能及黄酮醇支路的分流竞争对花色的影响提供基因资源和依据。
[Abstract]:Flavonol synthase (FLSs) is an important node enzyme. FLS gene expression not only affects flavonol synthesis, but also anthocyanin accumulation and anthocyanin color. In this study, a FLS gene, MaFLS1, was cloned from Hyacinth hyacinth (Hyacinth vinifera) by polymerase chain reaction (PCR). Sequence analysis showed that the full length of MaFLS1 cDNA was 1152 BP, encoding 383 amino acids. Homology comparison and evolution analysis showed that MaFLS1 belongs to 2-ketoglutaric acid and Fe2-dependent dioxygenase protein, and has a typical FLS protein domain, DHQ substrate specific binding sites, Fe2 and 2-ketoglutaric acid binding sites. Oil palm has the highest homology of FLS, which can reach 74%, which is related to Arabidopsis thaliana, Petunia and other model plants. Fluorescence quantitative PCR analysis showed that MaFLS1 gene was a non-tissue specific expression pattern in grape hyacinth, the highest expression level was in root, the second was in bulb and flower. The lowest expression level of MaFLS1 in leaves was significantly different in 5 different flower development stages of three different flower color grape hyacinth varieties, and was higher in the early stage of flower development of white variety 'Bailiren' (S 1 and S 2). The highest expression levels were observed in the pink cultivars' Pink Sunrise 'and the blue varieties' Armenia'in the late stage of S3 and S4. This study provided genetic resources and basis for further study on the function of FLS gene of Hyacinth grape in flower color presentation and the effect of fractionation competition of flavonol branch on flower color.
【作者单位】：西北农林科技大学风景园林艺术学院;旱区作物逆境生物学国家重点实验室(西北农林科技大学)/农业部西北地区园艺作物生物学与种质创制重点实验室;
【基金】：国家自然科学基金项目(31471905) 西北农林科技大学基本科研业务费科技创新专项(Z109021564)
【分类号】：S682.29

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