缺氧和充氧栽培对水稻Cd积累及OsHMA2基因表达的影响

发布时间：2018-06-09 07:11

本文选题：水稻 + 缺氧　；参考：《华南农业大学学报》2017年05期

【摘要】：【目的】研究缺氧和充氧栽培对水稻重金属Cd的吸收和转运及OsHMA2基因在茎中表达的影响,为降低水稻中重金属的吸收和累积奠定基础。【方法】以水稻品种五丰优2168为试验材料,通过营养液缺氧和营养液充氧的水培盆栽试验,分析Cd质量浓度为0、0.6、1.2mg·L~(-1)时,Cd的吸收累积以及茎中OsHMA2基因表达水平。【结果】缺氧栽培条件下,水稻生长发育受Cd抑制作用不显著,而充氧栽培条件下水稻生长受到显著抑制,表现为根、地上部干质量显著下降。在0.6和1.2mg·L~(-1)Cd处理条件下,缺氧栽培的水稻根部和地上部Cd累积量均低于充氧栽培。2种栽培条件下,根部Cd累积量均随Cd处理浓度的增大而增大。水稻地上部对Cd的累积在缺氧栽培条件下,0.6和1.2mg·L~(-1)Cd处理无显著差异,充氧栽培的水稻地上部Cd累积随处理浓度增大而增大。与对照相比,水稻茎OsHMA2表达量在缺氧和充氧栽培下,0.6mg·L~(-1)Cd处理上调,而1.2mg·L~(-1)Cd处理下调;在Cd处理下,OsHMA2表达量缺氧栽培高于充氧栽培。【结论】缺氧栽培能抑制水稻对Cd的吸收和累积;Cd累积量达到一定值时能够下调OsHMA2的表达。
[Abstract]:[objective] to study the effects of hypoxia and oxygenation on the uptake and transport of heavy metal CD in rice and the expression of OsHMA2 gene in stems. In order to reduce the absorption and accumulation of heavy metals in rice, the rice variety Wufengyou 2168 was used as the experimental material. The absorption and accumulation of CD and the expression level of OsHMA2 gene in stems were analyzed when CD concentration was 0. 6 ~ 1. 2mg / L ~ (-1). [results] under anoxic cultivation, the growth and development of rice was not inhibited by CD, but the growth of rice was significantly inhibited under aerobic cultivation. The root and dry mass of the shoot decreased significantly. Under the conditions of 0. 6 and 1.2mg-1 ~ (-1) CD treatment, the CD accumulation in roots and shoots of anoxic rice was lower than that in aerobic cultivation of 2 species, and the CD accumulation in roots increased with the increase of CD concentration. There was no significant difference in CD accumulation in shoot of rice under anoxic condition. CD accumulation increased with the increase of the concentration of CD in rice cultured with oxygen. Compared with the control, the expression of OsHMA2 in rice stem was up-regulated under hypoxia and oxygenation, but down-regulated by 1.2mg. Under CD treatment, the expression of OsHMA2 in anoxic culture was higher than that in oxygenated culture. [conclusion] hypoxia cultivation can inhibit the uptake of CD and the accumulation of CD in rice to a certain value, and can down-regulate the expression of OsHMA2.
【作者单位】：暨南大学环境学院/广州市环境暴露与健康重点实验室/广东省环境污染控制与修复材料中心;
【基金】：国家自然科学基金(41401365) 中国博士后科学基金(2015T80939,2014M552282)
【分类号】：S511

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