小鼠毛囊不同生长周期中MITF下游色素相关基因的定位表达及相关性分析

发布时间：2018-06-10 07:03

本文选题：小鼠 + 小眼畸形转录因子　；参考：《中国生物化学与分子生物学报》2017年02期

【摘要】：小眼畸形转录因子(MITF)不仅是黑色素细胞发育、增殖和存活的必要调节因子,而且对调节相关酶和黑素体蛋白表达来确保黑色素产生具有至关重要的作用。MITF下游色素相关基因在小鼠毛囊生长周期中的表达及相关性仍有待研究。HE染色结果表明不同毛囊时期的小鼠毛囊呈现典型的组织形态学结构;免疫组织化学显示,MITF、GPNMB、OA1、TYR、TYRP2在不同毛囊生长周期中的毛基质及内外毛根鞘均有不同程度的阳性表达。黑色素测定结果表明,在毛囊生长初期和中期,碱性可溶性总黑色素(ASM)、真黑素(EM)以及褐黑素(PM)相对含量高于毛囊生长末期。蛋白免疫印迹结果表明,MITF、GPNMB、OA1、TYR、TYRP2在毛囊生长初期和中期蛋白质相对水平明显高于毛囊生长末期。实时荧光定量PCR结果表明,MITF、GPNMB、OA1、TYR、TYRP2、PMEL在毛囊生长初期和中期,mRNA相对表达量显著高于毛囊生长末期。在不同毛囊生长周期小鼠皮肤的MITF下游色素相关基因表达存在显著差异,表明上述因子在维持黑色素细胞色素生成是不可或缺的因素。
[Abstract]:Transcriptional factor (MITF) is not only a necessary regulatory factor for the development, proliferation and survival of melanocytes, but also an essential regulator for the development, proliferation and survival of melanocytes. Moreover, the expression and correlation of MITF downstream pigment related genes in mouse hair follicle growth cycle need to be studied in order to ensure melanin production by regulating the expression of related enzymes and melanosomal proteins. The results of HE staining still need to be studied. The mouse hair follicles at different hair follicles showed typical histomorphological structure. Immunohistochemistry showed that MITFN MBOA1 TYRP2 was positive in different hair matrix and inside and outside hair root sheaths in different growth cycles of hair follicles. The results of melanin determination showed that the relative contents of basic soluble total melanin (ASMN) and melanin (EM) and PMMA were higher in the early and middle stage of hair follicle growth than in the latter stage of hair follicle growth. The results of Western blot showed that the relative level of protein in the early and middle stage of hair follicle growth was significantly higher than that in the later stage of hair follicle growth. The results of real-time fluorescence quantitative PCR showed that the relative expression of TYRP2PMEL in the early and middle stage of hair follicle growth was significantly higher than that in the latter stage of hair follicle growth. There were significant differences in the expression of MITF downstream pigment related genes in the skin of mice with different hair follicle growth cycles, which indicated that the above factors were indispensable factors in maintaining melanocytochrome production.
【作者单位】：山西农业大学动物科技学院;河北征宇制药有限公司;
【基金】：国家高技术研究发展计划项目(“863”计划,No.2013AA102506) 山西农业大学创新团队建设计划项目(No.CXTD201201) 国家自然科学基金项目(No.31302049)资助~~
【分类号】：Q78

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