陆地棉NAC转录因子基因GhNAC6的克

发布时间：2018-06-10 14:12

本文选题：棉花 + NAC转录因子　；参考：《棉花学报》2017年02期

【摘要】：【目的】NAC(NAM-ATAF-CUC)家族是植物细胞内特有的1类转录因子家族,参与植物的抗逆过程。为了研究棉花抗逆的分子机制,本研究从陆地棉TM-1中克隆到1个NAC转录因子基因并研究了其功能。【方法】根据其序列同源性和进化分析结果,将该基因命名为GhNAC6,本文对GhNAC6进行了生物信息学和表达分析,并利用病毒诱导基因沉默技术在棉花中沉默GhNAC6的表达。【结果】GhNAC6全长为1629 bp,包含2个内含子,其中编码区为900 bp,编码1个相对分子质量为33.9×10~3、等电点为6.18的蛋白。GhNAC6启动子区段包含多个与诱导表达和组织特异表达相关的顺式作用元件。转录组数据分析结果表明,GhNAC6在棉花发育的不同时期具有时空表达差异性;实时荧光定量核酸扩增检测结果显示GhNAC6还受到植物激素水杨酸、乙烯利、茉莉酸甲酯和逆境胁迫低温、高温、高盐、伤口的诱导。干涉GhNAC6基因发现,降低GhNAC6基因的表达能增强棉花对盐胁迫的耐受性。【结论】研究结果表明GhNAC6可能参与了棉花发育、逆境响应和激素信号传导的过程。
[Abstract]:[objective] NACU NAM-ATAF-CUC family is a family of transcription factors that are unique to plant cells and participate in the process of plant stress resistance. In order to study the molecular mechanism of cotton stress resistance, a NAC transcription factor gene was cloned from upland cotton TM-1 and its function was studied. [methods] according to its sequence homology and evolutionary analysis, The gene was named GhNAC6. In this paper, the expression of GhNAC6 was analyzed by bioinformatics, and the expression of GhNAC6 was silenced in cotton by virus induced gene silencing technique. [results] GhNAC6 was 1629 BP in length and contained two introns. The coding region is 900 BP, which encodes a protein with a relative molecular weight of 33.9 脳 10 ~ (-3) and a isoelectric point of 6.18. The promoter region of .GhNAC6 contains a number of cis-acting elements related to inducible expression and tissue specific expression. The results of transcriptional data analysis showed that GhNAC6 had different expression in time and space at different stages of cotton development, and that GhNAC6 was also affected by plant hormones salicylic acid, ethephon, methyl jasmonate and low temperature under stress, the results of real-time fluorescence quantitative nucleic acid amplification showed that GhNAC6 was also subjected to low temperature. High temperature, high salt, wound induction. By interfering with GhNAC6 gene, it was found that decreasing the expression of GhNAC6 gene could enhance the tolerance of cotton to salt stress. [conclusion] the results suggest that GhNAC6 may be involved in cotton development, stress response and hormone signal transduction.
【作者单位】：河南大学生命科学学院/棉花生物学国家重点实验室/河南省植物逆境生物学重点实验室;
【基金】：棉花生物学国家重点实验室开放课题(CB2015A31,CB2016A23) 河南省教育厅项目(15A180028,15A180029) 河南大学科学研究基金(2015YBZR030,2015YBZR031)
【分类号】：S562

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