青钱柳多糖对高脂血症小鼠SOD、GSH-Px、CAT基因mRNA表达的影响

发布时间：2018-06-10 16:17

本文选题：青钱柳 + 多糖　；参考：《江苏农业科学》2017年04期

【摘要】：为研究青钱柳多糖对高脂血症小鼠抗脂质过氧化相关基因表达的影响,将建模成功的高脂血症小鼠随机分为青钱柳多糖高、中、低剂量组,辛伐他汀组,高脂模型组和空白对照组,其中前4组分别灌胃400、200、100 mg/kg青钱柳多糖水溶液,4 mg/kg辛伐他汀水溶液,另2组灌胃等量无菌水,每组16只,连续喂养4周。试验结束后采用逆转录PCR(RT-PCR)测定肝脏、脂肪组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)基因的mRNA表达水平,探讨青钱柳多糖抗脂质过氧化机制。结果表明,不同剂量青钱柳多糖可使SOD、GSH-Px、CAT mRNA表达量显著或极显著上升(P0.05或P0.01),其中肝脏组织中SOD、CAT表达量最大增幅分别达130.9%、108.6%,GSH-Px表达量未出现显著变化;脂肪组织中SOD、GSH-Px的最大上调幅度分别为50.7%、100.0%,而CAT表达量无显著差异。结果显示,青钱柳多糖可调节高脂血症小鼠抗氧化相关基因的表达,增强其机体抗脂质过氧化能力。
[Abstract]:In order to study the effect of Salix mongolicum polysaccharides on the expression of anti-lipid peroxidation related genes in hyperlipidemic mice, hyperlipidemic mice were randomly divided into three groups: high, middle, low dose, simvastatin group. In the hyperlipidemia model group and the blank control group, the first four groups were fed with simvastatin solution for 400200100 mg/kg and simvastatin solution for 4 mg/kg, and the other two groups were fed with the same amount of aseptic water, 16 rats in each group for 4 weeks. The mRNA expression levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-PxX) and catalase (catalase) in liver and adipose tissue were determined by reverse transcription-PCR- RT-PCR. The results showed that the expression of GSH-PxPX cat mRNA was significantly or extremely significantly increased by different doses of Cyclocycline Polysaccharide, especially in liver tissue, the largest increase in the expression of SODH-PxPx was 130.9% and 108.6%, respectively. There was no significant change in the expression of GSH-Px in liver tissue. The maximum up-regulation of GSH-Px in adipose tissue was 50.7 and 100.0, respectively, but there was no significant difference in cat expression. The results showed that Polysaccharide could regulate the expression of antioxidant related genes in hyperlipidemic mice and enhance its ability of anti-lipid peroxidation.
【作者单位】：江西农业大学南昌市农产品加工与质量控制重点实验室;
【基金】：国家自然科学基金(编号:31560459、31160319) 江西省高等学校科技落地计划(编号:KJLD13027) 江西省自然科学基金(编号:20151BAB204037) 江西省教育厅科技计划(编号:GJJ13281)
【分类号】：R285.5

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