胡萝卜S-腺苷甲硫氨酸脱羧酶SAMDC基因的克隆及其对非生物胁迫的响应

发布时间：2018-06-11 11:12

本文选题：S-腺苷甲硫氨酸脱羧酶 + 克隆　；参考：《植物生理学报》2017年03期

【摘要】：S-腺苷甲硫氨酸脱羧酶是多胺生物合成过程中的关键酶,对于植物生长发育和抵御逆境胁迫等过程具有重要作用。本研究利用RT-PCR方法从胡萝卜品种‘黑田五寸’中克隆得到一个编码S-腺苷甲硫氨酸脱羧酶的基因Dc SAMDC。序列分析显示,该基因包含一个全长1 086 bp的开放阅读框,编码361个氨基酸。预测其蛋白质相对分子质量为40.16 kDa,理论等电点为4.89。胡萝卜S-腺苷甲硫氨酸脱羧酶具有高度保守的酶原剪切位点和PEST结构域。进化分析表明,胡萝卜SAMDC与葡萄的进化关系最为接近。荧光定量PCR分析显示,胡萝卜Dc SAMDC基因在叶片和根中的表达水平较高,对高温(38oC)、低温(4oC)、模拟干旱(200 g·L~(-1) PEG)和盐渍(200 g·L~(-1) Na Cl)胁迫有响应,并且,响应的时间较为迅速,在胁迫1~4 h后表达水平升至最高。本研究结果表明,Dc SAMDC基因可能在胡萝卜抵御非生物胁迫的过程中发挥重要作用。
[Abstract]:S-adenosine methionine decarboxylase is a key enzyme in polyamine biosynthesis and plays an important role in plant growth and development and resistance to stress. In this study, a gene encoding S- adenosine methionine decarboxylase (DC) was cloned from carrot variety 'black field five inch' by RT-PCR. Sequence analysis showed that the gene contained a 1 086 BP open reading frame encoding 361 amino acids. The predicted molecular weight of the protein is 40.16 kDa and the theoretical isoelectric point is 4.89. Carrot S- adenosine methionine decarboxylase has highly conserved proenzyme shear sites and pest domains. Evolutionary analysis showed that the evolutionary relationship between carrot SAMDC and grape was the most close. Fluorescence quantitative PCR analysis showed that the expression of DC SAMDC gene in leaves and roots of carrot was high, and it was responsive to high temperature (38oC), low temperature (4o C), simulated drought (200g / L) PEG) and saline (200g / L) NaCl. and the response time was relatively rapid. The expression level increased to the highest level after 4 hours of stress. The results suggested that DC SAMDC gene may play an important role in resisting abiotic stress in carrot.
【作者单位】：淮阴工学院生命科学与食品工程学院;南京农业大学园艺学院作物遗传与种质创新国家重点实验室农业部华东地区园艺作物生物学与种质创制重点实验室;
【基金】：淮阴工学院博士科研启动基金(Z301B16531) 教育部新世纪优秀人才支持计划项目(NCET-11-0670) 江苏省自然科学基金杰出青年基金(BK20130027)~~
【分类号】：S631.2;Q943.2

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