转基因细胞株BaF3-RAE1ε诱导产生的MDSC的杀伤功能及对NK细胞功能的影响

发布时间：2018-06-13 03:26

本文选题：视黄酸早期转录因子ε + 髓系抑制性细胞　；参考：《中国肿瘤生物治疗杂志》2017年08期

【摘要】：目的:探讨表达NKG2D配体视黄酸早期转录因子1ε(retinoic acid early transcript 1ε,RAE1ε)的原B细胞株BaF3诱导产生的髓系抑制性细胞(myeloid-derived suppressor cell,MDSC)的杀伤功能及对NK细胞功能的影响。方法:以小鼠原B细胞株BaF3为基础,构建表达空质粒的BaF3-mock对照细胞和表达RAE1ε的BaF3-RAE1ε细胞。将BaF3-mock和BaF3-RAE1ε细胞分别注射小鼠后诱导产生CD11b~+Gr-1~+MDSC,磁珠分选MDSC后与NK细胞共培养后,流式细胞术检测其对NK细胞表面NKG2D和CD107a表达的影响,ELISA法检测其对NK细胞分泌IFN-γ的影响。将MDSC分别与BaF3-mock和BaF3-RAE1ε细胞共培养后,乳酸脱氢酶释放法检测其对靶细胞BaF3-mock和BaF3-RAE1ε细胞的杀伤作用。结果:与BaF3-mock细胞相比,BaF3-RAE1ε细胞诱导产生的MDSC对NK细胞表面NKG2D和CD107a的表达没有明显影响(P0.05),对NK细胞分泌IFN-γ的水平也没有显著影响(P0.05)。与BaF3-mock细胞相比,BaF3-RAE1ε细胞诱导产生的MDSC对靶细胞BaF3-mock和BaF3-RAE1ε的杀伤作用明显增强[(1.99±0.39)%vs(8.63±1.45)%、(5.09±0.67)%vs(17.33±0.41)%,P0.01]。[(1.20±0.09)%vs(10.31±0.69)%、(5.52±1.64)%vs(18.91±3.04)%,P0.01]。结论:RAE-1ε增强MDSC对靶细胞的杀伤功能。
[Abstract]:Objective: To explore the killing function of myeloid suppressor cells (myeloid-derived suppressor cell, MDSC) induced by the original B cell line BaF3 expressing the early transcription factor 1 (retinoic acid early transcript 1 e, RAE1 E) and the effect on the function of the cells. BaF3-mock control cells of empty plasmids and BaF3-RAE1 epsilon cells expressing RAE1 epsilon. BaF3-mock and BaF3-RAE1 epsilon cells were injected into mice to induce CD11b~+Gr-1~+MDSC, and after MDSC was co cultured with NK cells, the effect on the expression of NKG2D and CD107a on the surface of NK cells was detected by flow cytometry. After coculture of MDSC with BaF3-mock and BaF3-RAE1 epsilon, the cytotoxicity of BaF3-mock and BaF3-RAE1 epsilon cells in target cells was detected by lactic dehydrogenase release method after co culture with BaF3-mock and BaF3-RAE1 epsilon cells. Results: compared with BaF3-mock cells, MDSC induced by BaF3-RAE1 e cells did not significantly affect the expression of NKG2D and CD107a in NK cells. There was no significant effect on the level of IFN- gamma secreted by NK cells (P0.05). Compared with BaF3-mock cells, the killing effect of MDSC on BaF3-mock and BaF3-RAE1 epsilon produced by BaF3-RAE1 cells was significantly enhanced [1.99 + 0.39)%vs (8.63 + 1.45)%, (5.09 + 0.67)%vs (17.33 + 0.41)%, P0.01].[(1.20 + 0.09)%vs (10.31 + 0.69)%, (5.52 + 1.64). (18.91 + 3.04)%, P0.01]. conclusion: RAE-1 MDSC enhances the killing function of target cells.
【作者单位】：扬州大学医学院病原生物学与免疫学教研室;
【基金】：国家自然科学基金资助项目(No.81373130,No.81001308) 江苏省自然科学基金资助项目(No.BK2010315) 扬州大学大学生创新创业训练计划项目~~
【分类号】：R730.51

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