非生物逆境胁迫下普通小麦烟农19幼苗FeSOD基因表达分析

发布时间：2018-06-13 20:00

  本文选题：普通小麦烟农 + 幼苗　； 参考：《作物杂志》2016年04期

【摘要】：非生物逆境(高温、低温、干旱、盐胁迫等)严重影响作物生长,研究参与逆境胁迫应答基因具有重要的理论意义和应用价值。超氧化物歧化酶(superoxide dismutase,SOD)是一种广泛存在于真核生物中的金属酶类,在植物的抗逆性中起到重要作用。采用q RT-PCR技术,分析普通小麦烟农19幼苗叶中Fe SOD基因在盐、脱落酸(ABA)、干旱、高温、低温胁迫过程中的的表达模式。结果表明:在逆境胁迫下,普通小麦烟农19幼苗Fe SOD基因的表达量大体呈现先上升后下降的趋势。在37℃高温、-4℃低温、300mmol/L Na Cl、30%的PEG-6000和100μmol/L ABA胁迫下,Fe SOD基因的表达量分别在3、3、6、48和24h时最高,分别为对照的34.0、4.6、4.3、5.8、13.5和3.3倍,差异均达到显著水平,说明Fe SOD基因在普通小麦烟农19幼苗逆境胁迫中发挥着重要的调控功能,为进一步了解小麦抗逆分子机制和改良小麦品种提供理论依据。
[Abstract]:Abiotic stresses (high temperature, low temperature, drought, salt stress, etc.) seriously affect crop growth. It is of great theoretical significance and practical value to study the genes involved in stress response. Superoxide dismutase (SOD) is a kind of metalloenzymes widely found in eukaryotes, which plays an important role in plant stress resistance. The expression patterns of Fe SOD gene in leaves of common wheat Yannong 19 seedlings were analyzed by using qRT-PCR during salt, abscisic acid, drought, high temperature and low temperature stress. The results showed that the expression of Fe SOD gene in common wheat Yannong 19 seedlings increased firstly and then decreased under stress. Under the stress of 30% PEG-6000 and 100 渭 mol / L ABA at 37 鈩，

本文编号：2015237