低分子质量壳寡糖对蛋鸡生产性能、蛋品质、血清生化指标、盲肠微生物数量及脾脏白细胞介素-2、肿瘤坏死因子-α基因表达的影响

发布时间：2018-06-13 23:21

本文选题：蛋鸡 + 壳寡糖　；参考：《动物营养学报》2017年05期

【摘要】：本试验旨在研究饲粮中添加不同水平的低分子质量(1 000 u)壳寡糖对蛋鸡生产性能、蛋品质、血清生化指标、盲肠微生物数量以及脾脏白细胞介素-2(IL-2)和肿瘤坏死因子-α(TNF-α)基因表达的影响。选取体重和产蛋率相近的58周龄海兰褐壳蛋鸡600只,随机分为4组,每组5个重复,每个重复30只。对照组饲喂基础饲粮,试验组分别饲喂在基础饲粮中添加300、600、900 mg/kg壳寡糖的试验饲粮。预试期为7 d,正试期为42 d。结果表明:1)添加300、600和900 mg/kg壳寡糖组的产蛋率分别比对照组提高了4.52%(P0.05)、2.99%(P0.05)和4.08%(P0.05)。2)试验第3、6周末,添加600和900 mg/kg壳寡糖组的鸡蛋哈夫单位分别比对照组提高了6.87%、6.69%和6.47%、6.60%(P0.05)。3)与对照组相比,饲粮添加600、900 mg/kg壳寡糖显著降低了血清葡萄糖、胆固醇含量和谷草转氨酶活性(P0.05)。4)与对照组相比,饲粮添加600、900 mg/kg壳寡糖显著提高了盲肠双歧杆菌和乳酸杆菌的数量(P0.05),显著降低了盲肠金黄色葡萄球菌的数量(P0.05)。5)与对照组相比,饲粮添加300、600 mg/kg壳寡糖显著提高了脾脏IL-2 m RNA表达水平(P0.05),饲粮添加600 mg/kg壳寡糖显著提高了脾脏TNF-αm RNA表达水平(P0.05)。由此可见,饲粮中添加不同水平的壳寡糖,提高了蛋鸡的产蛋率和哈夫单位,调节了肠道微生物菌群,增强了蛋鸡的免疫力,适宜壳寡糖添加水平为600 mg/kg。
[Abstract]:The aim of this study was to study the effects of different levels of low molecular weight oligosaccharide (LMWO) on performance, egg quality and serum biochemical indexes of laying hens. The effect of cecum microorganism quantity and spleen interleukin-2 (IL-2) and tumor necrosis factor- 伪 (TNF- 伪) gene expression. Six hundred Hailan brown shell hens of 58 weeks old with similar body weight and laying rate were randomly divided into 4 groups with 5 replicates in each group and 30 in each group. The control group was fed with basic diet and the experimental group was fed with 300600900 mg/kg chitosan oligosaccharide. The pretest period was 7 days and the positive test period was 42 days. The results showed that the egg production rate of the 300600 and 900 mg/kg chitosan oligosaccharide groups increased by 4.52% and 4.08% respectively compared with the control group (P 0.05) and 4.08 mg/kg chitosan oligosaccharide group at the end of the 3rd week of the experiment. The egg Hafu units of the 600 and 900 mg/kg chitosan oligosaccharide groups were increased by 6.87 ~ 6.69% and 6.47 ~ (6. 60) P ~ (0.05N) 路3, respectively, as compared with the control group, and compared with the control group, the egg production rate of the 300600 and 900 mg/kg chitosan oligosaccharides group was higher than that of the control group, and the egg production rate of the control group was higher than that of the control group. Dietary supplementation of 600900 mg/kg chitosan significantly decreased serum glucose, cholesterol and activity of glutamic oxaloacetic transaminase (P0.05. 4) compared with the control group. The amount of bifidobacterium cecum and lactobacillus was significantly increased and the quantity of staphylococcus aureus in cecum was decreased significantly by adding 600900 mg/kg chitosan oligosaccharide to diet.) compared with the control group, the quantity of staphylococcus aureus in cecum was significantly decreased. The level of IL-2 mRNA expression in spleen was significantly increased by adding 300600 mg/kg chitosan oligosaccharide to the diet, and the level of TNF- 伪 mRNA expression in spleen was significantly increased by adding 600 mg/kg chitosan oligosaccharide to the diet. It can be seen that adding different levels of chitosan oligosaccharide to diet can improve laying rate and Hafu unit of laying hens, regulate intestinal microbial flora, and enhance the immunity of laying hens. The suitable level of chitosan oligosaccharide addition is 600 mg / kg / kg.
【作者单位】：山东农业大学动物科技学院;泰山医学院;
【基金】：动物营养国家重点实验室开放课题项目(2004DA125184F1008)
【分类号】：S831.5

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