陆地棉KFB基因家族全基因组鉴定与表达分析

发布时间：2018-06-16 10:27

  本文选题：陆地棉 + KFB基因家族　； 参考：《棉花学报》2017年03期

【摘要】：【目的】KFB(Kelch containing F-box protein)是普遍存在于各种生物体中含有F-box结构域的一类家族蛋白,参与众多生物过程。本研究旨在开展陆地棉KFB家族基因鉴定和表达分析。【方法】利用生物信息学方法从陆地棉(TM-1)基因组中鉴定KFB家族成员,与已知的拟南芥、水稻、大豆等KFB蛋白序列构建系统进化树;进一步针对第Ⅱ亚族进行基因结构、染色体定位和表达分析。【结果】从陆地棉基因组共鉴定出150个KFB家族成员,进化分析表明这些基因可分为7个亚族。陆地棉KFB第Ⅱ亚族24个基因分布在14条染色体上,有9对直系同源基因;该亚族基因结构简单,半数基因只有1个外显子;qRT-PCR结果表明,该亚族基因具有较为广泛的组织表达模式,但在不同组织的表达存在差异。【结论】这些结果显示陆地棉KFB第Ⅱ亚族基因在进化过程中出现了功能分化。
[Abstract]:[objective] KFBK Kelch containing F-box protein is a kind of family protein which contains F-box domain in a variety of organisms and is involved in many biological processes. The purpose of this study was to identify and express the KFB gene of Upland cotton. [methods] the KFB family members were identified from the genome of TM-1 by bioinformatics, and the KFB family members were identified with the known Arabidopsis thaliana and rice. Phylogenetic tree was constructed from KFB protein sequences of soybean, and gene structure, chromosomal location and expression analysis were carried out for subfamily 鈪，

本文编号：2026348