香青兰总黄酮对载脂蛋白E基因缺陷小鼠动脉粥样硬化病变形成的影响

发布时间：2018-06-17 03:19

本文选题：香青兰总黄酮 + 动脉粥样硬化　；参考：《中国动脉硬化杂志》2017年09期

【摘要】：目的研究香青兰总黄酮对载脂蛋白E基因缺陷小鼠动脉粥样硬化病变的作用及可能的机制。方法载脂蛋白E基因缺陷小鼠随机分为模型组、辛伐他汀组及香青兰总黄酮高、中、低剂量组,以C57BL/6J小鼠为正常对照组,给药第12周后处死,检测血清血脂水平,主动脉粥样硬化斑块面积/管腔面积比值,免疫组织化学染色观察主动脉壁细胞间黏附分子1、血管细胞黏附分子1及增殖细胞核抗原的表达。结果与模型组比较,各给药组可以显著降低血清甘油三酯、低密度脂蛋白胆固醇水平(P0.05或P0.01);苏木素伊红染色观察给药组动脉粥样硬化病变减轻,斑块面积/管腔面积比值减小(P0.05或P0.01);免疫组织化学染色显示给药组细胞间黏附分子1、血管细胞黏附分子1及增殖细胞核抗原的表达下调(P0.05或P0.01)。结论香青兰总黄酮可能通过调节载脂蛋白E基因缺陷小鼠血脂代谢及下调主动脉壁细胞间黏附分子1、血管细胞黏附分子1及增殖细胞核抗原表达等的共同作用与影响,阻止载脂蛋白E基因缺陷小鼠动脉粥样硬化病变形成的发生、发展。
[Abstract]:Objective to study the effect and possible mechanism of total flavonoids of Cyclobalanthin on atherosclerosis in mice with apolipoprotein E gene deficiency. Methods mice with apolipoprotein E gene deficiency were randomly divided into model group, simvastatin group and high, middle and low dose groups of total flavonoids of Cyclobalanthin. C57BL / 6J mice were used as normal control group. After 12 weeks of administration, serum lipid levels were measured. The ratio of atherosclerotic plaque area to lumen area and the expression of intercellular adhesion molecule 1, vascular cell adhesion molecule 1 and proliferating cell nuclear antigen (PCNA) in aortic wall were observed by immunohistochemical staining. Results compared with the model group, the serum triglyceride, low density lipoprotein cholesterol (LDL-C) level and hematoxylin eosin staining were significantly decreased in each administration group, and the atherosclerotic lesion was alleviated by hematoxylin eosin staining. The ratio of plaque area to lumen area was decreased by P0.05 or P0.01, and the expression of intercellular adhesion molecule 1, vascular cell adhesion molecule 1 and proliferating cell nuclear antigen (PCNA) was down-regulated (P0.05 or P0.01). Conclusion Total flavonoids of Cyclobalanthus sinensis may play a role in regulating lipid metabolism and down-regulating the expression of intercellular adhesion molecule 1, vascular cell adhesion molecule 1 and proliferating cell nuclear antigen (PCNA) in apolipoprotein E deficient mice. To prevent the development of atherosclerosis in mice with apolipoprotein E deficiency.
【作者单位】：石河子大学医学院第一附属医院;石河子大学医学院;
【基金】：国家自然科学基金项目(81460638;81660704;81360631)
【分类号】：R285.5

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