凡纳滨对虾碱性磷酸酶和酸性磷酸酶基因的克

发布时间：2018-06-17 21:43

本文选题：凡纳滨对虾(Litopenaeus + vannamei)　；参考：《海洋科学》2017年01期

【摘要】：本研究通过c DNA末端快速扩增法(RACE)首次克隆得到凡纳滨对虾(Litopenaeus vannamei)碱性磷酸酶(alkaline phosphatase,ALP)和酸性磷酸酶(acid phosphatase,ACP)基因的c DNA全长序列,其中ALP基因全长序列1 910 bp,编码536个氨基酸;ACP基因全长序列1 544 bp,编码439个氨基酸。盐度调控设3个盐度组:31(对照)、16和3。养殖45 d后,荧光定量PCR测定对虾肝胰腺、肌肉、胃、肠和鳃5个组织中的ALP和ACP基因m RNA的表达情况。RT-PCR显示,ALP基因在5个组织中普遍表达,在肝胰腺中表达量最高(P0.05),肠道中的表达量最低(P0.05);ACP基因在5个组织中也均表达,在肝胰腺和鳃中表达量明显高于其他3个组织(P0.05)。盐度应答实验表明,不同盐度条件下,不同组织中,ALP和ACP基因存在不同的表达模式。在胃和鳃组织中,ALP基因在31盐度组的表达量显著高于其他两组(P0.05);在肝胰腺和肠道中,ALP基因在16盐度下的表达量明显低于其他两组(P0.05);在肌肉中,ALP基因的表达与其他组织均不同,31盐度组表达量最高,而16盐度组最低(P0.05);在肝胰腺中,ACP基因在31盐度组的表达量明显低于其他盐度组(P0.05);在肌肉和鳃两个组织中,ACP基因在31盐度下的表达量明显高于其余两个盐度组(P0.05);在肠道中,ACP基因的表达量在3盐度组明显高于其他两组(P0.05);而在胃中,ACP基因在3个盐度下的表达量没有显著性差异。上述结果为研究低盐条件下凡纳滨对虾的磷酸酶基因的变化机制提供了参考。
[Abstract]:In this study, the cDNA sequences of the alkaline phosphatase (ALP) and acid phosphatase (ACP) gene of Litopenaeus vannamei) were cloned for the first time by rapid amplification of cDNAs. The total length of ALP gene was 1 910 BP, the total length of ACP gene encoding 536 amino acids was 1 544 BP, and that of ALP gene was 439 amino acids. The salinity regulation was divided into 3 salinity groups: 1: 31 (control group: 16 and 3. After 45 days of culture, the expression of ALP and ACP mRNA in the liver and pancreas, muscle, stomach, intestine and Gill of prawn was detected by fluorescence quantitative PCR. RT-PCR showed that ALP gene was widely expressed in 5 tissues. The highest expression of P0.05 was found in the hepatopancreas, and the lowest expression of P0.05ACP was found in the intestine. The expression of ACP in the hepatopancreas and gills was significantly higher than that in the other three tissues. The salinity-response experiments showed that there were different expression patterns of ALP and ACP genes in different tissues under different salinity conditions. The expression of ALP gene in gastric and Gill tissues was significantly higher in the 31 salinity group than in the other two groups; in the hepatopancreas and intestine, the expression of the ALP gene in the liver, pancreas and intestine was significantly lower than that in the other two groups at 16 salinity; in the muscle, the ALP gene expression was similar to that in the other two groups. The highest expression level was observed in all tissues with different salinity. The expression of ACP gene in the hepatopancreas was significantly lower than that in the other salinities group, and the expression of ACP gene in the muscle and Gill tissues was significantly higher than that in the other two salinities groups (P0.05A), while the expression of ACP gene in the hepatopancreas was significantly lower than that in the other two salinities groups (P 0.05), while the expression of ACP gene in the muscle and Gill tissues was significantly higher than that in the other two salinity groups. The expression of ACP gene in the intestine was significantly higher in the 3 salinity group than in the other two groups, but there was no significant difference in the expression of ACP gene in the stomach under the three salinity conditions. These results provide a reference for studying the mechanism of phosphatase gene change in Penaeus vannamei under low salt condition.
【作者单位】：中国海洋大学水产学院;中国科学院实验海洋生物学重点实验室;青岛海洋科学与技术国家实验室海洋生物学与生物技术功能实验室;
【基金】：国家自然科学基金项目(41271547,41401644) 中国科学院战略性先导科技专项(XDA05010400)~~
【分类号】：S917.4

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