杨树NAC7转录因子基因应答盐胁迫表达

发布时间：2018-06-18 09:03

本文选题：小黑杨 + 基因表达　；参考：《东北林业大学学报》2017年08期

【摘要】：从小黑杨中克隆915 bp的NAC7转录因子基因(Potri.007G099400.1)cDNA,其编码304氨基酸,该蛋白属热稳定性较高的亲水性蛋白。用RT-qPCR分析杨树盐胁迫条件下NAC7基因表达情况,表明该基因对盐胁迫具有应答反应,且基因主要在根部表达。克隆获得1 062 bp的NAC7基因启动子DNA序列,其中含有许多胁迫应答元件,其驱动的GUS报告基因主要集中在根中表达,而在茎和叶中的表达很少,这个结果与NAC7基因表达进行RT-qPCR分析结果一致。
[Abstract]:A 915bp NAC7 transcription factor gene, Potri.007G099400.1, was cloned from Populus nigra. It encodes 304 amino acids and belongs to a hydrophilic protein with high thermal stability. The expression of NAC7 gene in poplar under salt stress was analyzed by RT-qPCR. The results showed that NAC7 gene was responsive to salt stress and mainly expressed in roots. A 1 062 BP DNA sequence of the promoter of NAC7 gene was obtained, which contained many stress response elements. The Gus reporter gene driven by NAC7 gene was mainly expressed in roots, but not in stems and leaves. The results were consistent with the results of RT-qPCR analysis of NAC7 gene expression.
【作者单位】：林木遗传育种国家重点实验室(东北林业大学);
【基金】：国家“863”课题(2013AA102701)
【分类号】：Q943.2;S792.11

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