冠突曲霉AN1类锌指蛋白zfpA基因的克隆及表达分析

发布时间：2018-06-18 18:27

本文选题：茯砖茶 + 有性产孢　；参考：《基因组学与应用生物学》2017年09期

【摘要】：本研究采用cDNA末端快速扩增技术(rapid amplification of cDNA ends,RACE)克隆出冠突曲霉中zfpA基因的cDNA全长序列并进行生物信息分析。该序列全长1 443 bp,开放阅读框长度为924 bp,从312~1 235 bp,没有内含子序列。推测编码的蛋白质含有307个氨基酸,同源性分析显示该基因与米曲霉的AN1锌指蛋白(XP_001826567)相似性达71%。利用荧光定量PCR技术分析了zfpA基因在营养菌丝体阶段、无性产孢阶段及有性产孢阶段的表达量差异。结果表明,这个基因在在无性产孢时期的表达量最低,有性产孢时期的表达量最高,相比无性产孢时期上调了4倍。本实验为深入研究zfpA基因对冠突曲霉的产孢调控机制奠定了基础。
[Abstract]:In this study, the full-length cDNA sequence of zfpA gene from Aspergillus coronatum was cloned by rapid amplification of cDNAs RACEE and the biological information was analyzed. The length of the sequence was 1 443 BP, the length of the open reading frame was 924 BP, and the length of the open reading frame was 1 235 BP from 312 to 1 235 BP, with no intron sequence. The deduced protein contained 307 amino acids. Homology analysis showed that the similarity between the gene and AN1 zinc finger protein XP001826567 of Aspergillus oryzae was 71%. The difference of zfpA gene expression in vegetative mycelia stage asexual sporulation stage and sexual sporulation stage was analyzed by fluorescence quantitative polymerase chain reaction (FQ-PCR). The results showed that the expression of this gene was the lowest in asexual sporulation stage and the highest in sexual sporulation stage, which was 4 times higher than that in asexual sporulation stage. This study laid a foundation for further study on the sporulation regulation mechanism of zfpA gene on Aspergillus coronatum.
【作者单位】：贵州大学农学院;贵州省生物技术研究所;贵州省农业生物技术重点实验室;贵州省农业科学院;
【基金】：贵州省联合基金(黔科合LH字(2014)7688号) 贵州省科技基础条件平台专项计划(黔科平台(2015)4007)基金共同资助
【分类号】：Q78;Q93

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